Anti-Deamidated Gliadin Peptide IgA ELISA Kit (DGP) (ab178616)

Overview

  • Product name
    Anti-Deamidated Gliadin Peptide IgA ELISA Kit (DGP)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sera 16 <=5.8%
    Inter-assay
    Sample n Mean SD CV%
    Sera <=10.2%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Indirect
  • Sensitivity
    > 0.74 U/ml
  • Range
    15 U/ml - 2400 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Deamidated Gliadin Peptide (DGP) IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgA class antibodies against deamidated Gliadin peptides (DGP) in Human serum and plasma.


    A 96-well plate has been precoated with deamidated Gliadin peptide (DGP) antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized deamidated Gliadin peptide (DGP)-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of deamidated Gliadin peptide (DGP) IgA sample captured in plate.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Cover Foil 1 unit
    Deamidated Gliadin Peptide Anti-IgA HRP Conjugate 1 x 15ml
    Deamidated Gliadin Peptide Coated Microplate (12 x 8 wells) 1 unit
    Negative Control 1 x 1.2ml
    Positive Control 1 x 1.2ml
    Anti-DGP Standard 0 - 0 U/mL 1 x 1.2ml
    Anti-DGP Standard 1 - 15 U/mL 1 x 1.2ml
    Anti-DGP Standard 2 - 30 U/mL 1 x 1.2ml
    Anti-DGP Standard 3 - 60 U/mL 1 x 1.2ml
    Anti-DGP Standard 4 - 240 U/mL 1 x 1.2ml
    IgA Sample Diluent 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Relevance
    Anti-gliadin antibodies are produced in response to gliadin, a prolamin found in wheat. In bread wheat it is encoded by three different alleles, AA, BB, and DD. These alleles can produce slightly different gliadins, which can cause the body to produce different antibodies. Some of these antibodies can detect proteins in specific grass taxa such as Triticeae (Triticeae glutens), while others react sporadically with certain species in those taxa, or over many taxonomically defined grass tribes. The IgA antibody is found in ~80% of patients with coeliac disease. It is directed against the alpha/beta and gamma (a,ß,?) gliadins. It is also found in a number of patients who are not enteropathic. Some of these patients may have neuropathies that respond favorably to a gluten elimination diet. This is referred to as gluten-sensitive idiopathic neuropathy. Clinically these antibodies and IgG antibodies to gliadin are abbreviated as AGA. The IgG antibody is similar to AGA IgA, but is found at higher levels in patients with the IgA-less phenotype. It is also associated with coeliac disease and idiopathic gluten sensitivity. Anti-gliadin antibodies are frequently found with anti-transglutaminase antibodies. The IgE antibodies are more typically found in allergy-related conditions such as urticaria, asthma, and wheat-dependent exercise-induced anaphylaxis. The target of the most allergenic antibodies is ?-5 gliadin, which is encoded by the Gli-1B gene found on the B haplome (Aegilops speltoides derived) of wheat.

Images

  • Representative Standard Curve using ab178616

Protocols

References

ab178616 has not yet been referenced specifically in any publications.

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