Key features and details
- Mouse monoclonal [5E8E7] to Decorin
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Decorin antibody [5E8E7]
See all Decorin primary antibodies
DescriptionMouse monoclonal [5E8E7] to Decorin
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee
- Human Decorin recombinant protein; Decorin (aa: 263-324)-hIgGFc transfected HEK293 cell lysate; HEK293 cells.
This product was changed from ascites to supernatant. Lot no’s high than GR150953-11 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer (Amino acid =85%, pH(1% water solution) =7.0~7.5, Water =2%, As(mg/kg) =0.5, Pb(mg/kg) =0.1).
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab181456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/200 - 1/4000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 40 kDa.|
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
Anti-Decorin antibody [5E8E7] (ab181456) at 1/500 dilution + Human Decorin recombinant protein
Predicted band size: 40 kDa
Expected MWt is 32.5 kDa.
All lanes : Anti-Decorin antibody [5E8E7] (ab181456) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Decorin (aa: 263-324)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 40 kDa
Flow cytometric analysis of HEK293 cells labeling Decorin with ab181456 at 1/200 dilution (green), compared to a negative control (red).
ab181456 has been referenced in 1 publication.
- Miguélez-Rivera L et al. Immunomodulation of mesenchymal stem cells in discogenic pain. Spine J 18:330-342 (2018). Flow Cyt . PubMed: 28939169