Anti-Decorin antibody (ab137508)
Key features and details
- Rabbit polyclonal to Decorin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Decorin antibody
See all Decorin primary antibodies -
Description
Rabbit polyclonal to Decorin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Dog, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 147-345 of Human Decorin (Uniprot ID: P07585).
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Positive control
- Recombinant Human Decorin protein (ab132145) can be used as a positive control in WB. 293T and Mouse heart cell lysates; paraformaldehyde fixed A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 280760 Cow
- Entrez Gene: 1634 Human
- Entrez Gene: 13179 Mouse
- Entrez Gene: 396957 Pig
- Entrez Gene: 100009171 Rabbit
- Omim: 125255 Human
- SwissProt: P21793 Cow
- SwissProt: P07585 Human
see all -
Alternative names
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
see all
Images
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Anti-Decorin antibody (ab137508) at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Anti-Decorin antibody (ab137508) at 1/1000 dilution + Mouse heart whole cell lysate
at 50 µg
Predicted band size: 40 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labelling Decorin, with ab137508 at 1/200 dilution.
The lower image was merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab137508 has been referenced in 8 publications.
- Shibata S et al. Role of Decorin in Posterior Capsule Opacification and Eye Lens Development. Cells 10:N/A (2021). PubMed: 33918979
- Zhang K et al. SIRT1 protects against aortic dissection by regulating AP-1/decorin signaling-mediated PDCD4 activation. Mol Biol Rep 47:2149-2159 (2020). PubMed: 32072402
- Dye BK et al. Smooth Muscle a-Actin Expression in Mitral Valve Interstitial Cells is Important for Mediating Extracellular Matrix Remodeling. J Cardiovasc Dev Dis 7:N/A (2020). PubMed: 32824919
- Jiang R et al. Kaempferol protects chondrogenic ATDC5 cells against inflammatory injury triggered by lipopolysaccharide through down-regulating miR-146a. Int Immunopharmacol 69:373-381 (2019). PubMed: 30776646
- Chu H et al. Effects of transplanted adipose derived stem cells on the expressions of a-SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears. Exp Ther Med 16:1729-1734 (2018). PubMed: 30186394
- Liu MM et al. Development and Evaluation of a Tissue-Engineered Fibrin-based Canine Mitral Valve Three-dimensional Cell Culture System. J Comp Pathol 160:23-33 (2018). IHC-P ; Dog . PubMed: 29729718
- Yates CC et al. Multipotent stromal cells/mesenchymal stem cells and fibroblasts combine to minimize skin hypertrophic scarring. Stem Cell Res Ther 8:193 (2017). PubMed: 28874184
- Murthi P et al. Decorin expression is decreased in first trimester placental tissue from pregnancies with small for gestation age infants at birth. Placenta 45:58-62 (2016). PubMed: 27577711