Key features and details
- Rabbit polyclonal to Decorin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Decorin antibody
See all Decorin primary antibodies
DescriptionRabbit polyclonal to Decorin
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 1-192 of Human Decorin.
- 293T and Raji cell lysates; TOV112D xenograft; A549 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
Immunohistochemical analysis of paraffin-embedded TOV112D xenograft labeling Decorin with ab151988 at 1/100 dilution.
Anti-Decorin antibody (ab151988) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed A549 cells labeling Decorin with ab151988 at 1/200 dilution. Lower panel shows co-staining with Hoechst 33342.
ab151988 has been referenced in 2 publications.
- Tanaka T et al. Association of matrix metalloproteinase-9 and decorin expression with the infiltration of cervical cancer. Oncol Lett 17:1306-1312 (2019). PubMed: 30655899
- Ono YJ et al. Decorin induced by progesterone plays a crucial role in suppressing endometriosis. J Endocrinol 223:203-16 (2014). WB, IHC-P ; Human . PubMed: 25244916