Overview

  • Product name

  • Description

    Goat polyclonal to Decorin
  • Host species

    Goat
  • Specificity

    ab189364 is expected to recognize the three reported isoforms a, b and c (NP_001911.1; NP_598011.1; NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Decorin aa 232-246 (internal sequence) (Cysteine residue).
    Sequence:

    KISRVDAASLKGLNN


    Database link: P07585

  • Positive control

    • Human breast tissue, Human kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    May affect the rate of fibrils formation.
  • Involvement in disease

    Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bone proteoglycan II antibody
    • CSCD antibody
    • DCN antibody
    • DCN protein antibody
    • Decorin antibody
    • Decorin proteoglycan antibody
    • Dermatan sulphate proteoglycans II antibody
    • DKFZp686J19238 antibody
    • DSPG 2 antibody
    • DSPG2 antibody
    • PG 40 antibody
    • PG II antibody
    • PG S2 antibody
    • PG-S2 antibody
    • PG40 antibody
    • PGII antibody
    • PGS 2 antibody
    • PGS2 antibody
    • PGS2_HUMAN antibody
    • Proteoglycan core protein antibody
    • SLRR1B antibody
    • Small leucine rich protein 1B antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast tissue labeling Decorin with ab189364 at 3.75 µg/ml.

  • Anti-Decorin antibody (ab189364) at 0.5 µg/ml + Human kidney tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab189364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pulmonary valve leaflet)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Envision Flex Target Retrieval Solution High pH
Permeabilization
No
Specification
Pulmonary valve leaflet
Blocking step
Rabbit Serum (Normal) plus Rabbit F(ab) anti-Sheep IgG as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100µg/mL · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2016

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