Anti-Decorin antibody [DCN/3523] (ab268048)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [DCN/3523] (ab268048)](https://www.abcam.com/ps/products/268/ab268048/Images/ab268048-3-anti-dcn-antibody-dcn3523-immunohistochemistry-2.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [DCN/3523] (ab268048)")
Key features and details
- Mouse monoclonal [DCN/3523] to Decorin
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Decorin antibody [DCN/3523]
See all Decorin primary antibodies -
Description
Mouse monoclonal [DCN/3523] to Decorin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Decorin aa 212-336. The exact sequence is proprietary.
Database link: P07585 -
Positive control
- IHC-P: Human prostate and skin tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
DCN/3523 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab268048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Protein Array | Use at an assay dependent concentration. |
Target
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1634 Human
- Omim: 125255 Human
- SwissProt: P07585 Human
- Unigene: 728830 Human
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Alternative names
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [DCN/3523] (ab268048)
Formalin-fixed, paraffin-embedded human skin tissue stained for Decorin using ab268048 at 2 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [DCN/3523] (ab268048)](https://www.abcam.com/ps/products/268/ab268048/Images/ab268048-2-anti-dcn-antibody-dcn3523-immunohistochemistry-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [DCN/3523] (ab268048)Formalin-fixed, paraffin-embedded human prostate tissue stained for Decorin using ab268048 at 2 μg/ml in immunohistochemical analysis.
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![Protein Array - Anti-Decorin antibody [DCN/3523] (ab268048)](https://www.abcam.com/ps/products/268/ab268048/Images/ab268048-1-anti-dcn-antibody-dcn3523-protein-array-4.jpg)
Protein Array - Anti-Decorin antibody [DCN/3523] (ab268048)Protein Array containing more than 19,000 full-length human proteins using ab268048. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab268048 has not yet been referenced specifically in any publications.
