Key features and details
- Mouse monoclonal [DCN/3523] to Decorin
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Decorin antibody [DCN/3523]
See all Decorin primary antibodies
DescriptionMouse monoclonal [DCN/3523] to Decorin
Tested applicationsSuitable for: IHC-P, Protein Arraymore details
Species reactivityReacts with: Human
Recombinant fragment within Human Decorin aa 212-336. The exact sequence is proprietary.
Database link: P07585
- IHC-P: Human prostate and skin tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor concentrate
Light chain typekappa
Our Abpromise guarantee covers the use of ab268048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|Protein Array||Use at an assay dependent concentration.|
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Bone proteoglycan II antibody
- CSCD antibody
- DCN antibody
Formalin-fixed, paraffin-embedded human skin tissue stained for Decorin using ab268048 at 2 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate tissue stained for Decorin using ab268048 at 2 μg/ml in immunohistochemical analysis.
Protein Array containing more than 19,000 full-length human proteins using ab268048. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268048 has not yet been referenced specifically in any publications.