Anti-DECR2/PDCR antibody (ab153849)
Key features and details
- Rabbit polyclonal to DECR2/PDCR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DECR2/PDCR antibody -
Description
Rabbit polyclonal to DECR2/PDCR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
-
Immunogen
Recombinant fragment corresponding to Human DECR2/PDCR aa 92-242.
-
Positive control
- A549 whole cell lysate; HeLa, HepG2 and HCT116 cells; A549 xenograft
-
General notes
This product was previously labelled as DECR2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/500. |
Target
-
Function
Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. -
Cellular localization
Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 26063 Human
- SwissProt: Q9NUI1 Human
- Unigene: 628831 Human
- Unigene: 655999 Human
-
Alternative names
- 2 4 dienoyl CoA reductase 2 antibody
- 2 4 dienoyl CoA reductase 2 peroxisomal antibody
- 2 antibody
see all
Images
-
Western blot - Anti-DECR2/PDCR antibody (ab153849)Anti-DECR2/PDCR antibody (ab153849) at 1/1000 dilution + A549 (30 µg of whole cell lysate)
Predicted band size: 31 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DECR2/PDCR antibody (ab153849)Immunocytochemistry/ Immunofluorescence of A549 cells fixed in 4% paraformaldehyde staining DECR2/PDCR using ab153849 at a dilution of 1/500 (green). Nuclear staining with Hoechst 33342 staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR2/PDCR antibody (ab153849)Immunohistochemical analysis of paraffin-embedded A549 xenograft using ab153849 antibody at 1:100 dilution
Protocols
References (0)
ab153849 has not yet been referenced specifically in any publications.
