Anti-DECR2/PDCR antibody (ab153849)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DECR2/PDCR antibody
See all DECR2/PDCR primary antibodies -
Description
Rabbit polyclonal to DECR2/PDCR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment corresponding to Human DECR2/PDCR aa 92-242.
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Positive control
- A549 whole cell lysate; HeLa, HepG2 and HCT116 cells; A549 xenograft
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General notes
This product was previously labelled as DECR2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/500. |
Target
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Function
Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 26063 Human
- SwissProt: Q9NUI1 Human
- Unigene: 628831 Human
- Unigene: 655999 Human
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Alternative names
- 2 4 dienoyl CoA reductase 2 antibody
- 2 4 dienoyl CoA reductase 2 peroxisomal antibody
- 2 antibody
see all
Images
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Western blot - Anti-DECR2/PDCR antibody (ab153849)Anti-DECR2/PDCR antibody (ab153849) at 1/1000 dilution + A549 (30 µg of whole cell lysate)
Predicted band size: 31 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DECR2/PDCR antibody (ab153849)Immunocytochemistry/ Immunofluorescence of A549 cells fixed in 4% paraformaldehyde staining DECR2/PDCR using ab153849 at a dilution of 1/500 (green). Nuclear staining with Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR2/PDCR antibody (ab153849)Immunohistochemical analysis of paraffin-embedded A549 xenograft using ab153849 antibody at 1:100 dilution
References
ab153849 has not yet been referenced specifically in any publications.
