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    dectin-1-antibody-2a11-ab53427.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Dectin-1 antibody [2A11] (ab53427)

  • Datasheet
Submit a review Q&A (1)References (5)

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Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)

    Key features and details

    • Rat monoclonal [2A11] to Dectin-1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    FITC

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Protein
    Product image
    Recombinant Human Dectin-1 protein (ab163849)

    View more associated products

    Overview

    • Product name

      Anti-Dectin-1 antibody [2A11]
      See all Dectin-1 primary antibodies
    • Description

      Rat monoclonal [2A11] to Dectin-1
    • Host species

      Rat
    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: IHC-P or IHC-R
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      This product was produced with the following immunogens:
      Recombinant full length protein corresponding to Dectin-1. Recombinant soluble Dectin-1.

      Tissue, cells or virus. Dectin-1 transfected NIH3T3 cells

    • General notes


      ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Primary antibody notes

      ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
    • Clonality

      Monoclonal
    • Clone number

      2A11
    • Isotype

      IgG2b
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Neuroscience
      • Development

    Associated products

    • Alternative Versions

      • FITC Anti-Dectin-1 antibody [2A11] (ab21646)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
    • Recombinant Protein

      • Recombinant Human Dectin-1 protein (ab163849)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab53427 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/10 - 1/50.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.
    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

    Notes
    Flow Cyt
    1/10 - 1/50.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.
    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

    Application notes
    Is unsuitable for IHC-P or IHC-R.

    Target

    • Function

      Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
    • Tissue specificity

      Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
    • Involvement in disease

      Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues in response to glucan binding.
    • Cellular localization

      Cytoplasm and Cell membrane.
    • Target information above from: UniProt accession Q9BXN2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 56644 Mouse
      • SwissProt: Q6QLQ4 Mouse
      • Unigene: 239516 Mouse
      • Alternative names

        • Beta GR antibody
        • Beta-glucan receptor antibody
        • BGR antibody
        • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
        • C type lectin domain family 7 member a antibody
        • C-type lectin domain family 7 member A antibody
        • C-type lectin superfamily member 12 antibody
        • CANDF4 antibody
        • CLC7A_HUMAN antibody
        • CLEC7A antibody
        • CLEC7A protein antibody
        • Clecsf12 antibody
        • DC-associated C-type lectin 1 antibody
        • Dectin 1 antibody
        • DECTIN 1 receptor antibody
        • Dectin-1 antibody
        • Dectin1 antibody
        • Dendritic cell associated C type lectin 1 antibody
        • Dendritic cell-associated C-type lectin 1 antibody
        • Lectin like receptor 1 antibody
        • Putative transmembrane protein dectin 1 antibody
        see all

      Images

      • Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)
        Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)
        Staining with Rat anti mouse beta Glucan Receptor. (ab53427).

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab53427? Please let us know so that we can cite the reference in this datasheet.

      ab53427 has been referenced in 5 publications.

      • Barilla RM  et al. Specialized dendritic cells induce tumor-promoting IL-10+IL-17+ FoxP3neg regulatory CD4+ T cells in pancreatic carcinoma. Nat Commun 10:1424 (2019). PubMed: 30926808
      • Kim HW  et al. Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley. Int J Biol Macromol 131:1084-1091 (2019). PubMed: 30914368
      • Park HR  et al. Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels. Int J Biol Macromol 137:657-665 (2019). PubMed: 31276719
      • Juban G  et al. AMPK Activation Regulates LTBP4-Dependent TGF-ß1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep 25:2163-2176.e6 (2018). PubMed: 30463013
      • Seifert L  et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . PubMed: 26655905

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      This customer has highly interested in ab53427 (Anti-beta Glucan Receptor antibody [2A11]), he wants to know the epitope of this antibody, and could you please help this customer to solve the problem. I look forward to your kindly reply. Thanks for your kindly assistance.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 04 2012

      Answer

      Thank you for your inquiry.

      I am sorry the epitope of this antibody hasn't been determined yet. The immunogen of this antibody corresponds to recombinant beta Glucan Receptor protein.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

      Read More

      Abcam Scientific Support

      Answered on May 04 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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