Anti-Dectin-1 antibody [2A11] (ab53427)

Submit a review Q&A (1)References (5)

Overview

  • Product name

    Anti-Dectin-1 antibody [2A11]
    See all Dectin-1 primary antibodies

  • Description

    Rat monoclonal [2A11] to Dectin-1

  • Host species

    Rat

  • Tested applications

    Suitable for: IP, IHC-Fr, Flow Cytmore details
    Unsuitable for: IHC-P or IHC-R

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to Dectin-1.

  • General notes


    ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

  • Primary antibody notes

    ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

  • Clonality

    Monoclonal

  • Clone number

    2A11

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

The epitope recognised by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody.
Flow Cyt 1/10 - 1/50.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IHC-R.

    • Target

    • Function

      Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.

    • Tissue specificity

      Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.

    • Involvement in disease

      Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine residues in response to glucan binding.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Beta GR antibody
        • Beta-glucan receptor antibody
        • BGR antibody
        • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
        • C type lectin domain family 7 member a antibody
        • C-type lectin domain family 7 member A antibody
        • C-type lectin superfamily member 12 antibody
        • CANDF4 antibody
        • CLC7A_HUMAN antibody
        • CLEC7A antibody
        • CLEC7A protein antibody
        • Clecsf12 antibody
        • DC-associated C-type lectin 1 antibody
        • Dectin 1 antibody
        • DECTIN 1 receptor antibody
        • Dectin-1 antibody
        • Dectin1 antibody
        • Dendritic cell associated C type lectin 1 antibody
        • Dendritic cell-associated C-type lectin 1 antibody
        • Lectin like receptor 1 antibody
        • Putative transmembrane protein dectin 1 antibody
        see all

      Images

      • Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (ab53427)
        Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (ab53427)
        Staining with Rat anti mouse beta Glucan Receptor. (ab53427).

      References

      Publishing research using ab53427? Please let us know so that we can cite the reference in this datasheet.

      ab53427 has been referenced in 5 publications.

      • Barilla RM  et al. Specialized dendritic cells induce tumor-promoting IL-10+IL-17+ FoxP3neg regulatory CD4+ T cells in pancreatic carcinoma. Nat Commun 10:1424 (2019). PubMed: 30926808
      • Kim HW  et al. Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley. Int J Biol Macromol 131:1084-1091 (2019). PubMed: 30914368
      • Park HR  et al. Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels. Int J Biol Macromol 137:657-665 (2019). PubMed: 31276719
      • Juban G  et al. AMPK Activation Regulates LTBP4-Dependent TGF-ß1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep 25:2163-2176.e6 (2018). PubMed: 30463013
      • Seifert L  et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . PubMed: 26655905

      Customer reviews and Q&As

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      Question

      This customer has highly interested in ab53427 (Anti-beta Glucan Receptor antibody [2A11]), he wants to know the epitope of this antibody, and could you please help this customer to solve the problem. I look forward to your kindly reply. Thanks for your kindly assistance.

      Best regards

      Read More
      Answer

      Thank you for your inquiry.

      I am sorry the epitope of this antibody hasn't been determined yet. The immunogen of this antibody corresponds to recombinant beta Glucan Receptor protein.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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