Anti-Dectin-1 antibody [2A11] (ab53427)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Dectin-1 antibody [2A11]
See all Dectin-1 primary antibodies -
DescriptionRat monoclonal [2A11] to Dectin-1
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Host speciesRat
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Tested applicationsSuitable for: IP, IHC-Fr, Flow Cytmore details
Unsuitable for: IHC-P or IHC-R -
Species reactivityReacts with: Mouse
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Immunogen
Tissue, cells or virus corresponding to Dectin-1.
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General notes
ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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Primary antibody notesab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
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ClonalityMonoclonal
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Clone number2A11
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IsotypeIgG2b
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab53427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. The epitope recognised by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody. |
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| Flow Cyt | 1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Target
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FunctionLectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
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Tissue specificityHighly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
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Involvement in diseaseDefects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
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Sequence similaritiesContains 1 C-type lectin domain.
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Post-translational
modificationsPhosphorylated on tyrosine residues in response to glucan binding. -
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 56644 Mouse
- SwissProt: Q6QLQ4 Mouse
- Unigene: 239516 Mouse
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Alternative names
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
Images
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![Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (ab53427)](https://www.abcam.com/ps/datasheet/Images/53/ab53427/ab53427_1.jpg)
Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (ab53427)Staining with Rat anti mouse beta Glucan Receptor. (ab53427).
Datasheets and documents
References
This product has been referenced in:
- Seifert L et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . Read more (PubMed: 26655905) »
