Anti-Dectin-1 antibody [2A11] (ab53427)
![Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)](https://www.abcam.com/ps/products/53/ab53427/Images/ab53427-66203-anti-beta-glucan-receptor-antibody-2a11-flow-cytometry.jpg "Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)")
Key features and details
- Rat monoclonal [2A11] to Dectin-1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Dectin-1 antibody [2A11]
See all Dectin-1 primary antibodies -
Description
Rat monoclonal [2A11] to Dectin-1 -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P or IHC-R -
Species reactivity
Reacts with: Mouse -
Immunogen
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Dectin-1. Recombinant soluble Dectin-1.
Tissue, cells or virus. Dectin-1 transfected NIH3T3 cells -
General notes
ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Primary antibody notes
ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line. -
Clonality
Monoclonal -
Clone number
2A11 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/10 - 1/50.
Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Notes |
|---|
|
Flow Cyt
1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Target
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Function
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
Tissue specificity
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
Involvement in disease
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to glucan binding. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 56644 Mouse
- SwissProt: Q6QLQ4 Mouse
- Unigene: 239516 Mouse
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Alternative names
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
Images
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Flow Cytometry - Anti-Dectin-1 antibody [2A11] (ab53427)Staining with Rat anti mouse beta Glucan Receptor. (ab53427).
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab53427 has been referenced in 5 publications.
- Barilla RM et al. Specialized dendritic cells induce tumor-promoting IL-10+IL-17+ FoxP3neg regulatory CD4+ T cells in pancreatic carcinoma. Nat Commun 10:1424 (2019). PubMed: 30926808
- Kim HW et al. Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley. Int J Biol Macromol 131:1084-1091 (2019). PubMed: 30914368
- Park HR et al. Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels. Int J Biol Macromol 137:657-665 (2019). PubMed: 31276719
- Juban G et al. AMPK Activation Regulates LTBP4-Dependent TGF-ß1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep 25:2163-2176.e6 (2018). PubMed: 30463013
- Seifert L et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . PubMed: 26655905
