Overview

  • Product name

    Anti-Dectin-1 antibody [2A11] (FITC)
    See all Dectin-1 primary antibodies
  • Description

    Rat monoclonal [2A11] to Dectin-1 (FITC)
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Dectin-1.

  • General notes


    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
  • Clonality

    Monoclonal
  • Clone number

    2A11
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells in 100µl.

 

 

 

 

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • Involvement in disease

    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to glucan binding.
  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

Images

  • Flow cytometry staining of mouse peripheral blood granulocytes with ab21646.

References

This product has been referenced in:

  • Daley D  et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med 23:556-567 (2017). IHC-Fr, Flow Cyt ; Mouse . Read more (PubMed: 28394331) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer


Information we have from the clone originators indicates the antibody recognises both isoforms.

Read More
Application
Flow Cytometry
Sample
Mouse Cell (RAW and J774 murine macrophages)
Specification
RAW and J774 murine macrophages
Preparation
Cell harvesting/tissue preparation method: routine cell culture --> scraped, washed
Sample buffer: PBS + FBS (3%)
Fixation
None
Permeabilization
No
Gating Strategy
Size gating of murine RAW cells

Abcam user community

Verified customer

Submitted May 23 2008

Answer

Thank you for your enquiry. The antibody is specific for Dectin-1. This is the only antibody we carry for this particular target. The references listed on the datasheet are general references pertaining to the target of the antibody. They are not papers that use this particular antibody (in that case, they would be listed as "References that used ab21646", or something to that effect). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

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