Anti-Dectin-1 antibody [2A11] (FITC) (ab21646)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Dectin-1 antibody [2A11] (FITC)
See all Dectin-1 primary antibodies -
Description
Rat monoclonal [2A11] to Dectin-1 (FITC) -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein corresponding to Dectin-1.
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General notes
This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor. -
Clonality
Monoclonal -
Clone number
2A11 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab21646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use 10µl for 106 cells in 100µl.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
Tissue specificity
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
Involvement in disease
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to glucan binding. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 56644 Mouse
- SwissProt: Q6QLQ4 Mouse
- Unigene: 239516 Mouse
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Alternative names
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
Images
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![Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (FITC) (ab21646)](https://www.abcam.com/ps/products/21/ab21646/Images/ab21646-30690-anti-beta-glucan-receptor-antibody-2a11-fitc-flow-cytometry.jpg)
Flow Cytometry - Anti-beta Glucan Receptor antibody [2A11] (FITC) (ab21646)Flow cytometry staining of mouse peripheral blood granulocytes with ab21646.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Daley D et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med 23:556-567 (2017). IHC-Fr, Flow Cyt ; Mouse . Read more (PubMed: 28394331) »
