FITC Anti-Dectin-1 antibody [2A11] (ab21646)
Key features and details
- FITC Rat monoclonal [2A11] to Dectin-1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-Dectin-1 antibody [2A11]
See all Dectin-1 primary antibodies -
Description
FITC Rat monoclonal [2A11] to Dectin-1 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor. -
Clonality
Monoclonal -
Clone number
2A11 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use 10µl for 106 cells.
Use 10µl for 106 cells in 100µl.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 10µl for 106 cells. Use 10µl for 106 cells in 100µl.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
Tissue specificity
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
Involvement in disease
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to glucan binding. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 56644 Mouse
- SwissProt: Q6QLQ4 Mouse
- Unigene: 239516 Mouse
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Alternative names
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab21646 has been referenced in 1 publication.
- Daley D et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med 23:556-567 (2017). IHC-Fr, Flow Cyt ; Mouse . PubMed: 28394331