Overview

  • Product name

  • Description

    Rabbit polyclonal to Dectin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Dectin-1 aa 70-120 (extracellular). The exact sequence is proprietary.
    Database link: Q9BXN2-1

  • Positive control

    • Rat spleen tissue lysate; Human spleen tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • Involvement in disease

    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to glucan binding.
  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

Images

  • Immunofluorescence of beta Glucan receptor in Human spleen tissue with ab140039 at 20 ug/mL.
  • Lane 1 : Anti-Dectin-1 antibody (ab140039) at 1 µg/ml
    Lane 2 : Anti-Dectin-1 antibody (ab140039) at 2 µg/ml

    All lanes : Rat spleen tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue, labelling beta Glucan Receptor using ab140039 at 5 µg/ml.

References

This product has been referenced in:

  • Yang L  et al. Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway. J Ethnopharmacol 223:51-62 (2018). WB, IHC-P ; Mouse . Read more (PubMed: 29775695) »
  • Xia Y  et al. Dectin-1 predicts adverse postoperative prognosis of patients with clear cell renal cell carcinoma. Sci Rep 6:32657 (2016). Read more (PubMed: 27600310) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.

As discussed on the telephone, I can confirm that is covered by the guarantee for IHC-P. All tested applications and species covered by the guarantee are listed on the individual datasheets. Further information regarding the guarantee can be found on the following page from the website:

https://www.abcam.com/content/abpromise-guarantee

The laboratory have provided the requested IHC protocol. The citrate buffer for antigen retrieval is pH 5.5 and the antibody was used at 5ug/mL. Please note this is a guideline only and may require some individual optimization.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.



MATERIALS

Paraffin‐embedded tissue slides

Coverslips

Slide racks

Staining dishes with lids

Plastic slide tray (Baxter Scientific Cat. No. M6304 or similar)

Orbital shaker

PAP pen

Transfer pipettes

Deionized water (DI H2O)

PBS (Phosphate Buffered Saline)

Hydrogen peroxide (H2O2)

Primary antibody

Biotinylated secondary antibody, HRP conjugated

Bovine Serum Albumin (BSA – for blocking)

Streptavidin‐HRP

DAB

Hematoxylin (optional)

Acetic Acid (optional)

Paramount Coverslip solution

BUFFERS

Working Citrate Buffer

9mL of 0.1M Citric Acid (10.5g citric acid monohydrate to 500mL DI H2O)

41mL of 0.1M Sodium Citrate (14.7g sodium citrate dehydrate to 500mL DI H2O)

450mL of DI H2O

Deparaffinization and Rehydration (Cover staining dishes with a lid in each step)

1. Dip slides in three (3) changes of xylene or a xylene substitute for 3 minutes each.

2. Dip slides in two (2) change of 100% alcohol for 3 minutes each.

3. Dip slides in one (1) change of 95% alcohol for 3 minutes.

4. Dip slides in one (1) change of 70% alcohol for 3 minutes.

5. Rinse slides twice (2x) in DI H2O for 5 minutes.



Antigen Retrieval (Microwave Method)

6. Soak slides in 3% H2O2 for 5 minutes.

7. Rinse slides twice (2x) in DI H2O for 5 minutes.

8. Soak the slides in the working citrate buffer and cover with a lid.

9. Microwave until the liquid boils, about 1‐5 minutes.

10. Remove from heat and let it stand at room temperature for 20 minutes.

11. Wash three (3) times for 5 minutes in DI H2O

12. Remove the liquid (do not touch the tissue!) and use a PAP pen to circle around the tissue.


Blocking

13. Apply enough 5% BSA with a transfer pipette to cover the tissues.

14. Incubate the slides overnight at 4°C in a humid chamber.



Primary Antibody

15. Dilute the primary antibody to the recommended concentration in 1% BSA/PBS diluent.

16. Remove the BSA, and incubate with primary antibody solution for 1 hour at room temperature.

17. Wash slides three (3) times 5 minutes each on the shaker.

Secondary Antibody and Detection

18. Dilute the biotinylated secondary antibody to 1:200 in 1% BSA diluent.

19. Incubate with secondary antibody solution for 30 minutes at room temperature.

20. Wash slides in PBS three (3) times 5 minutes each on the shaker.

21. Add enough streptavidin HRP to cover the tissues. Incubate for 30 minutes at room temperature

22. Wash three (3) times 5 minutes each in PBS on the shaker.

23. Add enough DAB to cover the tissues. Once the cells start turning brown (inexperienced

technicians may wish to observe this under a microscope), wash twice (2x) in PBS for 5 minutes

each on the shaker.



Optional Counterstain

24. Dip the slide rack with the slides into a staining dish of hematoxylin for 30 seconds.

25. Dip into an acetic bath (200mL DI H2O with one to three drops of acetic acid). Rinse with DI H2O.

Dehydration (Cover staining dishes with a lid in each step)

26. Dip slides in 70% and 95% alcohol for 3 minutes each.

27. Dip slides in 2 changes of 100% alcohol for 3 minutes.

28. Dip slides in 3 changes of xylene or xylene substitute for 3 minutes.



Cover Slips

29. Drizzle Paramount coverslip solution onto coverslips or slides.

30. Apply coverslip to slide.

31. Let the slides dry overnight.

Read More

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