Anti-Dectin-1 antibody (ab140039)
Key features and details
- Rabbit polyclonal to Dectin-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dectin-1 antibody
See all Dectin-1 primary antibodies -
Description
Rabbit polyclonal to Dectin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Dectin-1 aa 70-120 (extracellular). The exact sequence is proprietary. This antibody was raised against a 17 amino acid synthetic peptide near the center of human Dectin-1.
Database link: Q9BXN2-1 -
Positive control
- WB: Rat spleen tissue lysate IHC-P: Human spleen tissue ICC/IF: Jurkat cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa.
WB for rat samples only. |
|
IHC-P |
Use a concentration of 5 µg/ml.
IHC-P suitable for Human samples only |
|
ICC/IF |
Use a concentration of 20 µg/ml.
ICC/IF suitable for Human samples only |
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa. WB for rat samples only. |
IHC-P
Use a concentration of 5 µg/ml. IHC-P suitable for Human samples only |
ICC/IF
Use a concentration of 20 µg/ml. ICC/IF suitable for Human samples only |
Target
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Function
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
Tissue specificity
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
Involvement in disease
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to glucan binding. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64581 Human
- Entrez Gene: 502902 Rat
- Omim: 606264 Human
- SwissProt: Q9BXN2 Human
- Unigene: 143929 Human
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Alternative names
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human spleen tissue, labelling Dectin-1 using ab140039 at 5 µg/ml.
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Lane 1 : Anti-Dectin-1 antibody (ab140039) at 1 µg/ml
Lane 2 : Anti-Dectin-1 antibody (ab140039) at 2 µg/ml
All lanes : Rat spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 28 kDa -
Immunocytochemistry/ Immunofluorescence analysis of Jurkat cells labeling Dectin-1 wiht ab140039 at 20µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab140039 has been referenced in 14 publications.
- Al Madhoun A et al. Dectin-1 as a Potential Inflammatory Biomarker for Metabolic Inflammation in Adipose Tissue of Individuals with Obesity. Cells 11:N/A (2022). PubMed: 36139454
- Ding Z et al. Inhibition of Dectin-1 Alleviates Neuroinflammatory Injury by Attenuating NLRP3 Inflammasome-Mediated Pyroptosis After Intracerebral Hemorrhage in Mice: Preliminary Study Results. J Inflamm Res 15:5917-5933 (2022). PubMed: 36274828
- Liu J et al. Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice. BMC Gastroenterol 22:469 (2022). PubMed: 36401221
- Zhang D et al. The Dectin-1 Receptor Signaling Pathway Mediates the Remyelination Effect of Lentinan through Suppression of Neuroinflammation and Conversion of Microglia. J Immunol Res 2022:3002304 (2022). PubMed: 36619719
- Ren A et al. Inhibition of Dectin-1 on Dendritic Cells Prevents Maturation and Prolongs Murine Islet Allograft Survival. J Inflamm Res 14:63-73 (2021). PubMed: 33469336