Key features and details
- Mouse monoclonal [IMG3D1] to DECTIN 2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human, Chimpanzee, Orangutan
- Isotype: IgG3
Product nameAnti-DECTIN 2 antibody [IMG3D1]
See all DECTIN 2 primary antibodies
DescriptionMouse monoclonal [IMG3D1] to DECTIN 2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human, Chimpanzee, Orangutan
Synthetic peptide corresponding to Human DECTIN 2 aa 75-139.
Database link: Q6EIG7
- Human, mouse and rat heart lysate, human Ramos cell lysate, human PBMCs and human spleen tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab107572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
Use 0.5 ug/5x105 cells
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
FunctionBinds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.
Tissue specificityExpressed in lung, spleen, lymph node, leukocytes, bone marrow, tonsils and dendritic cells. Strongly expressed in purified monocytes and weakly in B-cells. In peripheral blood cells, preferentially expressed in plasmacytoids rather than myeloids.
Sequence similaritiesContains 1 C-type lectin domain.
DomainA short stretch of the intracellular domain (AA 8-14) proximal to the transmembrane domain is required for association with Fc receptor gamma chain.
- Information by UniProt
- C type lectin domain family 6 member A antibody
- C type lectin superfamily member 10 antibody
- C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 10 antibody
Cell surface flow cytometric analysis of DECTIN 2 expression by 5x10^5 human PBMCs using 0.5 µg of ab107572 in red and 0.5 µg of isotype control in green.
All lanes : Anti-DECTIN 2 antibody [IMG3D1] (ab107572) at 2 µg/ml
Lane 1 : Human heart lysate
Lane 2 : Human heart lysate with immunizing peptide
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Lane 5 : Human Ramos cell lysate
All lanes : goat anti-mouse Ig HRP
Predicted band size: 24 kDa
Immunohistochemical analysis of DECTIN 2 in formalin-fixed, paraffin-embedded human spleen tissue using ab107572 at 5 µg/ml.
ab107572 has been referenced in 1 publication.
- Yan X et al. Dectin-2 Deficiency Modulates Th1 Differentiation and Improves Wound Healing After Myocardial Infarction. Circ Res 120:1116-1129 (2017). PubMed: 28193608