Overview

  • Product name

  • Description

    Rabbit polyclonal to DEDD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human DEDD (internal sequence). The exact sequence is proprietary.
    Database link: O75618

  • Positive control

    • ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function

    A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro.
  • Tissue specificity

    Widely expressed with highest levels in testis.
  • Sequence similarities

    Contains 1 DED (death effector) domain.
  • Post-translational
    modifications

    Exists predominantly in a mono- or diubiquitinated form.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Translocated to the nucleus during CD95-mediated apoptosis where it is localized in the nucleoli (By similarity). Following apoptosis induction, the mono and/or diubiquitination form increases and forms filamentous structures that colocalize with KRT8 and KRT18 intermediate filament network in simple epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • CASP8IP1 antibody
    • Death effector domain containing antibody
    • Death effector domain containing protein antibody
    • Death effector domain containing testicular molecule antibody
    • Death effector domain-containing protein antibody
    • Death effector domain-containing testicular molecule antibody
    • DEDD 1 antibody
    • dedD antibody
    • DEDD_HUMAN antibody
    • DEDD1 antibody
    • DEDPro 1 antibody
    • DEDPro1 antibody
    • DEFT antibody
    • FLDED 1 antibody
    • FLDED-1 antibody
    • FLDED1 antibody
    • KE 05 antibody
    • KE05 antibody
    • OTTHUMP00000031822 antibody
    see all

Images

  • 4% paraformaldehyde-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for DEDD (green) using ab227294 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

References

ab227294 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab227294.
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