Product nameAnti-DEDD2 antibody
DescriptionRabbit polyclonal to DEDD2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A peptide corresponding to 11 amino acids near the amino-terminus of human DEDD2.
- RAW264.7 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 36 kDa).|
RelevanceApoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified. Over-expression of DEDD2 in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, it has been shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.
Cellular localizationNuclear, accumulated in subnuclear structures resembling nucleoli.
- Death effector domain containing 2 antibody
- Death effector domain-containing DNA-binding protein antibody
- Death effector domain-containing protein antibody
Lane 1 : Anti-DEDD2 antibody (ab36858) at 0.5 µg/ml
Lane 2 : Anti-DEDD2 antibody (ab36858) at 1 µg/ml
Lane 3 : Anti-DEDD2 antibody (ab36858) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg/ml per lane.
All lanes : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Lanes read from left to right 1, 2 and 3.
ab36858 has not yet been referenced specifically in any publications.