Overview

  • Product name
    Anti-DEDD2 antibody
  • Description
    Rabbit polyclonal to DEDD2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A peptide corresponding to 11 amino acids near the amino-terminus of human DEDD2.

  • Positive control
    • RAW264.7 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 36 kDa).

Target

  • Relevance
    Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified. Over-expression of DEDD2 in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, it has been shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.
  • Cellular localization
    Nuclear, accumulated in subnuclear structures resembling nucleoli.
  • Database links
  • Alternative names
    • Death effector domain containing 2 antibody
    • Death effector domain-containing DNA-binding protein antibody
    • Death effector domain-containing protein antibody
    • Death effector domain-containing testicular protein antibody
    • DED containing protein FLAME 3 antibody
    • DEFT antibody
    • DNA binding death effector domain containing protein 2 antibody
    • DNA-binding death effector domain-containing protein 2 antibody
    • FADD like anti apoptotic molecule 3 antibody
    • FLAME 3 antibody
    see all

Images

  • Lane 1 : Anti-DEDD2 antibody (ab36858) at 0.5 µg/ml
    Lane 2 : Anti-DEDD2 antibody (ab36858) at 1 µg/ml
    Lane 3 : Anti-DEDD2 antibody (ab36858) at 2 µg/ml

    All lanes : RAW264.7 cell lysate

    Lysates/proteins at 15 µg/ml per lane.

    Secondary
    All lanes : Anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Lanes read from left to right 1, 2 and 3.

References

ab36858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

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The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Question
Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided; the protocol seems fine to me however I have following recommendations that might help to get good staining;

- try the antibody 5-10ug/ml.
- In our lab we use 5% BSA as blocking agent. Please try this.
- We in lab heat lysates for 10 minutes at 100C. Please try this step.
- Try no primary control

I am sure these suggestions will help to get nice clean band of correct size. Please do not hesitate to contact me for further assistance if the results does not improve.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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