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“We have recently purchased the antibody DEDD2 (ab36858) and have tried to detect the protein by western using the recommendations on the supplied spec sheet. Unfortunately an overnight incubation at final antibody concentration at 2ug/ml did not produce the expected result (26-36kDa band). However, after a lengthy 10min exposure a 70kDa band was observed. We are concerned this may be an alternative isoform (DEDD1). Could you please provide technical assistance for this product on how we can achieve the expected band. We loaded 25ug total protein per well."
I have attached a Technical Questioner completed by customer as well. This was purchased by Sapphire on our PO# PO-14403, dated 07/06/12.
Please let me know if you require any further information.
PS. I tried to lodge the inquiry online but it wouldn`t let me as it kept asking for the correct lot#. Please see screen shot below.
Asked on Jul 10 2012