For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Please find attached questionnaire as requested.
Asked on Jul 11 2012
Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided; the protocol seems fine to me however I have following recommendations that might help to get good staining;
- try the antibody 5-10ug/ml.
- In our lab we use 5% BSA as blocking agent. Please try this.
- We in lab heat lysates for 10 minutes at 100C. Please try this step.
- Try no primary control
I am sure these suggestions will help to get nice clean band of correct size. Please do not hesitate to contact me for further assistance if the results does not improve.
Answered on Jul 11 2012