Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7492] to DEF6
- Suitable for: WB, IP, ICC, Flow Cyt
- Reacts with: Human
Product nameAnti-DEF6 antibody [EPR7492]
See all DEF6 primary antibodies
DescriptionRabbit monoclonal [EPR7492] to DEF6
Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues on the C terminus in Human DEF6 (UniProt Q9H4E7).
- Human tonsil, Ramos, MOLT4, Human thymus, Raji and Jurkat lysates; permeabilized Jurkat cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.01 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IP||1/10 - 1/100.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
Tissue specificityBroadly expressed in the immune system and can be detected in T and B-cells.
Sequence similaritiesContains 1 PH domain.
DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
modificationsTyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
- Information by UniProt
- DEF-6 antibody
- Def6 antibody
- DEFI6_HUMAN antibody
All lanes : Anti-DEF6 antibody [EPR7492] (ab126792) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Ramos lysate
Lane 3 : MOLT4 lysate
Lane 4 : Human thymus lysate
Lane 5 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
ab126792 at 1/100 dilution staining DEF6 in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126792 has been referenced in 1 publication.
- Joshi RN et al. Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells. Front Immunol 8:1163 (2017). PubMed: 28993769