Overview

  • Product name

    Anti-DEF6 antibody [EPR7492]
    See all DEF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR7492] to DEF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus in Human DEF6 (UniProt Q9H4E7).

  • Positive control

    • Human tonsil, Ramos, MOLT4, Human thymus, Raji and Jurkat lysates; permeabilized Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.01 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7492
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
    • Tissue specificity

      Broadly expressed in the immune system and can be detected in T and B-cells.
    • Sequence similarities

      Contains 1 PH domain.
    • Domain

      The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • Post-translational
      modifications

      Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
    • Cellular localization

      Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
    • Information by UniProt
    • Database links

    • Alternative names

      • DEF-6 antibody
      • Def6 antibody
      • DEFI6_HUMAN antibody
      • Differentially expressed in FDCP 6 homolog antibody
      • Differentially expressed in FDCP 6 homologue mouse antibody
      • IBP antibody
      • IRF4-binding protein antibody
      • Swap70 like adapter of T cells antibody
      see all

    Images

    • All lanes : Anti-DEF6 antibody [EPR7492] (ab126792) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Ramos lysate
      Lane 3 : MOLT4 lysate
      Lane 4 : Human thymus lysate
      Lane 5 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 74 kDa

    • ab126792 at 1/100 dilution staining DEF6 in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Joshi RN  et al. Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells. Front Immunol 8:1163 (2017). Read more (PubMed: 28993769) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126792.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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