Overview

  • Product name

  • Description

    Rabbit polyclonal to DEFB-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Indirect ELISA, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human DEFB-4. Highly pure (>98%).

  • General notes

    Protein previously labeled as beta 4 Defensin.

Properties

  • Form

    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.5 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23627872
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml.

Can be paired for ELISA with Rabbit polyclonal to DEFB-4 (Biotin) (ab70216).

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to DEFB-4 (Biotin) (ab70216). Capture

Target

  • Function

    Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
  • Tissue specificity

    High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
  • Sequence similarities

    Belongs to the beta-defensin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BD 4 antibody
    • BD-4 antibody
    • BD4 antibody
    • beta 104 antibody
    • beta 4 Defensin antibody
    • Beta defensin 4 antibody
    • Beta-defensin 104 antibody
    • Beta-defensin 4 antibody
    • D104A_HUMAN antibody
    • DEFB-4 antibody
    • DEFB104B antibody
    • Defensin antibody
    • Defensin beta 104 antibody
    • Defensin beta 4 precursor antibody
    • hBD 4 antibody
    • hBD-4 antibody
    • hBD4 antibody
    see all

Images

  • ab70215 staining DEFB-4 in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor® 488-conjugated was used as the secondary antibody and DAPI used to stain the nuclei.

References

This product has been referenced in:

  • Pierson T  et al. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis 11:10 (2013). ICC/IF ; Human . Read more (PubMed: 23627872) »
See 1 Publication for this product

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