- References (4)
Product nameDeferoxamine mesylate, Iron chelator
- desferrioxamine B
Biological descriptionIron chelator. Blood-brain barrier permeable. Displays a number of biological actions such as immunomodulation, inhibition of inflammation and atherosclerosis, and reduction of neuronal death.
Chemical nameN4-[5-[[4-5-(Acetylhydroxyamino)pentyl]-1,4-dioxobutyl]hydroxyaminopentyl-N1-(5-aminopentyl)-N1-hydroxybutanediamide methanesulfonate
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Titration of HeLa-DFO extract within the working range of the assay. Background subtracted data from duplicate measurements are plotted. To induce HIF1 alpha protein levels, HeLa cells were treated with 500 µM DFO (ab120727) for 24 hours.
Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements.
Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by SimpleStep ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by SimpleStep ELISA (40 µg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both SimpleStep ELISA and western blot.
This product has been referenced in:
- Zhang WJ et al. The iron chelator, desferrioxamine, reduces inflammation and atherosclerotic lesion development in experimental mice. Exp Biol Med (Maywood) 235:633-41 (2010). Read more (PubMed: 20463304) »
- Okauchi M et al. Effects of deferoxamine on intracerebral hemorrhage-induced brain injury in aged rats. Stroke 40:1858-63 (2009). Read more (PubMed: 19286595) »
- Choi EY et al. Iron chelator triggers inflammatory signals in human intestinal epithelial cells: involvement of p38 and extracellular signal-regulated kinase signaling pathways. J Immunol 172:7069-77 (2004). Read more (PubMed: 15153529) »