Recombinant
RabMAb

Anti-Deformed Epidermal Autoregulatory Factor 1 antibody [EPR10322] (ab156591)

Overview

  • Product name
    Anti-Deformed Epidermal Autoregulatory Factor 1 antibody [EPR10322]
    See all Deformed Epidermal Autoregulatory Factor 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10322] to Deformed Epidermal Autoregulatory Factor 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human Deformed Epidermal Autoregulatory Factor 1 (UniProt O75398).

  • Positive control
    • 293T, Jurkat, SH-SY5Y and HeLa cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.
    • Tissue specificity
      Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.
    • Sequence similarities
      Contains 1 MYND-type zinc finger.
      Contains 1 SAND domain.
    • Cellular localization
      Cytoplasm. Nucleus. When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced; Nucleus. Cytoplasm. Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% and Secreted. Secreted in some cell types.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deaf1 antibody
      • DEAF1 transcription factor antibody
      • DEAF1_HUMAN antibody
      • Deformed epidermal autoregulatory factor 1 homolog antibody
      • Nuclear DEA1 related transcriptional regulator antibody
      • Nuclear DEAF-1-related transcriptional regulator antibody
      • NUDR antibody
      • SPN antibody
      • Suppressin antibody
      • Zinc finger MYND domain containing protein 5 antibody
      • Zinc finger MYND domain-containing protein 5 antibody
      • ZMYND5 antibody
      see all

    Images

    • All lanes : Anti-Deformed Epidermal Autoregulatory Factor 1 antibody [EPR10322] (ab156591) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunofluorescent analysis of HeLa cells labeling Deformed Epidermal Autoregulatory Factor 1 with ab156591 at 1/100 dilution.

    References

    ab156591 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab156591.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up