Product nameAnti-DEGA antibody
See all DEGA primary antibodies
DescriptionRabbit polyclonal to DEGA
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- IHC-P: Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
Tissue specificityHighest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
Sequence similaritiesBelongs to the immunoglobulin superfamily. AMIGO family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Cellular localizationCell membrane. Nucleus. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons.
- Information by UniProt
- Adhesion molecule with Ig like domain 2 antibody
- ALI 1 antibody
- ALI1 antibody
Paraffin-embedded formalin-fixed human kidney tissue stained for DEGA using ab272658 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-DEGA antibody (ab272658) at 0.4 µg/ml
Lane 1 : Vector only transfected into HEK293T cells (Human epithelial cell line from embryonic kidney transformed with large T antigen).
Lane 2 : AMIGO2 over-expression in HEK293T cells
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272658 has not yet been referenced specifically in any publications.