Overview

  • Product name

  • Description

    Rabbit polyclonal to DEGA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DEGA (C terminal).
    Database link: NM_181847

  • Positive control

    • Human breast carcinoma tissue.
  • General notes

    Previously labelled as AMIGO2. 

Properties

  • Form

    Lyophilised:Reconstitute in 200ul sterile H2O.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab84416 is by a peptide affinity column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC/IF 1/100.

Target

  • Function

    Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
  • Tissue specificity

    Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. AMIGO family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Cellular localization

    Cell membrane. Nucleus. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion molecule with Ig like domain 2 antibody
    • ALI 1 antibody
    • ALI1 antibody
    • Alivin 1 antibody
    • Alivin-1 antibody
    • Alivin1 antibody
    • AMGO2_HUMAN antibody
    • AMIGO 2 antibody
    • AMIGO-2 antibody
    • Amigo2 antibody
    • Amphoterin induced gene 2 antibody
    • Amphoterin induced protein 2 antibody
    • Amphoterin-induced gene and open reading frame 2 antibody
    • Amphoterin-induced protein 2 antibody
    • DEGA antibody
    • Differentially expressed in gastric adenocarcinoma antibody
    • Differentially expressed in gastric adenocarcinomas antibody
    • Transmembrane protein AMIGO 2 antibody
    • Transmembrane protein AMIGO2 antibody
    see all

Images

  • ab84416 at 1/100 dilution staining DEGA in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References

ab84416 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing (gradient 4-12% NuPAGE)
Loading amount
30 µg
Specification
melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 12 2016

Answer

Thank you for contacting us.
Your credit note ID is *****.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Answer

Thank you for your reply.

I would be happy to raise a discount code for testing ab84416 in Mouse samples with WB if you could please use Human fetal brain lysate or Human breast carcinoma tissue as positive control.

As two untested variants would be used, running a known positive control is essential in case the antibody does not recognize the protein in such assay.

Please let me know if you would still be interested, and I will then send you the mentioned code.

Thank you for your cooperation.

Read More

Answer

Thank you for your reply.

I am happy to hear you are interested in participating in our Abreview system. However, ab84416 has not been tested in WB and unfortunately the discount code is only applicable for only one untested species / applications.

The discount code is valid for testing ab84416 in Mouse with ELISA, IHC-P or ICC/IF. Could you please confirm in which applications you would be interested in testing it?

Thank you for your cooperation. I look forward to hearing from you.

Read More

Answer

Thank you for your patience.

Both anti-AMIGO antibodies (ab84416 and ab84476) share the same immunogen sequence. In fact the difference between both antibodies is the purification process. While ab84476 is provided as whole antiserum, ab84416 has been purified by immunogen affinity.

The likelihood to cross react with Mouse species is the same in both cases, so you could participate in the Abreview system testing either of them in Mouse samples. Please notice ab84416 has been tested in ELISA, IHC-P and ICC/IF whereas ab84476 has been used in WB and ELISA.

Please let me know which antibody you would like to test in Mouse species and I will be happy to raise a discount code for you.

I look forward for receiving your reply.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human umbilical vein endothelial cells)
Permeabilization
Yes - triton X-100
Specification
Human umbilical vein endothelial cells
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 21 2011

Answer

DISCOUNT CODE: xxxxx Expiration date: 2012-01-06 I am very pleased to hear you would like to accept our offer and test ab84416 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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