Overview

  • Product name

  • Description

    Rabbit polyclonal to DEK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human DEK aa 325-375. The exact sequence is proprietary. NP_003463.1
    Database link: P35659

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human stomach carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245430 was affinity purified using an epitope specific to DEK immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in chromatin organization.
    • Tissue specificity

      Ubiquitous. Expressed at relatively high levels.
    • Involvement in disease

      A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
    • Sequence similarities

      Contains 1 SAP domain.
    • Post-translational
      modifications

      Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
    • Cellular localization

      Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
    • Information by UniProt
    • Database links

    • Alternative names

      • D6S231E antibody
      • Dek antibody
      • DEK gene antibody
      • DEK oncogene antibody
      • DEK oncogene DNA binding antibody
      • DEK_HUMAN antibody
      • Protein DEK antibody
      see all

    Images

    • All lanes : Anti-DEK antibody (ab245430) at 0.04 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Exposure time: 10 seconds
    • Formalin-fixed, paraffin-embedded human stomach carcinoma tissue stained for DEK with ab245430 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

    References

    ab245430 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245430.
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