Recombinant Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2380] to delta 1 Catenin/CAS (phospho S268)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [EPR2380] to delta 1 Catenin/CAS (phospho S268) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho S268).
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Positive control
- HeLa cell lysate treated with Calyculin A. A431 cells. Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2380 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
Domain
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
Post-translational
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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Alternative names
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
Images
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All lanes : Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted? -
Immunofluorescent staining of delta 1 Catenin/CAS in A431 cells using ab79545 at 1/100 dilution.
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ab79545 at 1/100 dilution staining delta 1 Catenin/CAS in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab79545 has been referenced in 1 publication.
- Alharatani R et al. Novel truncating mutations in CTNND1 cause a dominant craniofacial and cardiac syndrome. Hum Mol Genet 29:1900-1921 (2020). PubMed: 32196547