The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa).
Use at 2-5 µg/mg of lysate.
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Expressed in vascular endothelium.
Belongs to the beta-catenin family. Contains 10 ARM repeats.
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling delta 1 Catenin with ab72039 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - Anti-delta 1 Catenin antibody (ab72039)
All lanes : Anti-delta 1 Catenin antibody (ab72039) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of delta 1 Catenin by Western Blot of Immunprecipitate.
ab72039 at 1µg/ml staining delta 1 Catenin in HeLa whole cell lysate immunoprecipitated using ab72039 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.