Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit polyclonal to delta 1 Catenin/CAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 625-675. The exact sequence is proprietary. NP_001322.1
    Database link: O60716

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245403 was affinity purified using an epitope specific to delta 1 Catenin/CAS immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificity

      Expressed in vascular endothelium.
    • Sequence similarities

      Belongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • Domain

      A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications

      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localization

      Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for delta 1 Catenin/CAS using ab245403 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • delta 1 Catenin/CAS was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245403 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-delta 1 Catenin/CAS antibody at 1 µg/ml.

      Lane 1: ab245403 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab245403 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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