Product nameAnti-delta 1 Catenin/CAS antibody
See all delta 1 Catenin/CAS primary antibodies
DescriptionRabbit polyclonal to delta 1 Catenin/CAS
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Rhesus monkey
- This antibody gave a positive signal in the following Lysates: HeLa Nuclear Whole Cell EBS E14 TG2A Day 0 Whole Cell
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family.
Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
All lanes : Anti-delta 1 Catenin/CAS antibody (ab71138) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 50 kDa, 56 kDa, 68 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
ICC/IF image of ab71138 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71138, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
ab71138 has not yet been referenced specifically in any publications.