Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit polyclonal to delta 1 Catenin/CAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human delta 1 Catenin/CAS aa 75-125.
    Database link: NP_001322.1

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.

Epitope retrieval with Tris-EDTA pH 9.0 is recommended.

WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain

    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications

    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • delta 1 Catenin antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Images

  • All lanes : Anti-delta 1 Catenin/CAS antibody (ab72039) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling delta 1 Catenin/CAS with ab72039 at 1/1000 (0.2µg/ml). Detection: DAB.

  • Detection of delta 1 Catenin/CAS by Western Blot of Immunprecipitate.
    ab72039 at 1µg/ml staining delta 1 Catenin/CAS in HeLa whole cell lysate immunoprecipitated using ab72039 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.

References

This product has been referenced in:

  • Lenin R  et al. GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability. Sci Rep 9:10783 (2019). Read more (PubMed: 31346222) »
See 1 Publication for this product

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