Recombinant Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-delta 1 Catenin/CAS antibody [EPR357(2)]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS -
Host species
Rabbit -
Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human delta 1 Catenin/CAS aa 950 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK-293T, RAW264.7, C2C12 and A431 cell lysates. IHC-P: Human breast carcinoma, ovarian cancer and colonic adenocarcinoma tissues. ICC/IF: A431 cells. Flow Cytometry: A431 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR357(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 108 kDa. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
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| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/20. |
Target
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Function
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
Domain
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
Post-translational
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
- Unigene: 35738 Mouse
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Alternative names
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
Images
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![Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-349223-anti-delta-1-catenin-cas-antibody-epr357-2-westernblot-human-mouse.jpg)
Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)All lanes : Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?The multiple bands are isoforms caused by alternatively splicing as was described in PMID 9653641 and are consistent with what has been descirbed in PMID 15077190.
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![Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-7-anti-delta-1-catenin-cas-antibody-epr357-2-immunocytochemistry-a431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-6-anti-delta-1-catenin-cas-antibody-epr357-2-immunohistochemistry-ovarian-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-107602-anti-delta-1-catenin-cas-antibody-epr3572-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-107603-anti-delta-1-catenin-cas-antibody-epr3572-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-268996-anti-delta-1-catenin-cas-antibody-epr3572-flow-cytometry.jpg)
Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Other - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)](https://www.abcam.com/ps/products/92/ab92514/Images/ab92514-107601-anti-delta-1-catenin-cas-antibody-epr3572.jpg)
Other - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Raghu D et al. GALNT3 Maintains the Epithelial State in Trophoblast Stem Cells. Cell Rep 26:3684-3697.e7 (2019). Read more (PubMed: 30917321) »
- An Y et al. Cdh1 and Pik3ca Mutations Cooperate to Induce Immune-Related Invasive Lobular Carcinoma of the Breast. Cell Rep 25:702-714.e6 (2018). Read more (PubMed: 30332649) »
