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  1. Link

    delta-1-catenincas-antibody-epr3572-ab92514.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins
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RecombinantRabMAb

Recombinant Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (15)

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Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
  • Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [EPR357(2)]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 950 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK-293T, RAW264.7, C2C12 and A431 cell lysates. IHC-P: Human breast carcinoma, ovarian cancer and colonic adenocarcinoma tissues. ICC/IF: A431 cells. Flow Cyt (intra): A431 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR357(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Associated products

  • Alternative Versions

    • Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
    • A-431 membrane extract lysate (ab30134)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Flow Cyt (Intra)
1/20.
ICC/IF (3)
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Flow Cyt (Intra)
1/20.
ICC/IF
1/100 - 1/250.

Target

  • Function

    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain

    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications

    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Target information above from: UniProt accession O60716 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1500 Human
    • Entrez Gene: 12388 Mouse
    • Omim: 601045 Human
    • SwissProt: O60716 Human
    • SwissProt: P30999 Mouse
    • Unigene: 166011 Human
    • Unigene: 35738 Mouse
    • Alternative names

      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Images

    • Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      All lanes : Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
      Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
      Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 108 kDa
      Observed band size: 70-120 kDa why is the actual band size different from the predicted?



      The multiple bands are isoforms caused by alternatively splicing  as was described in PMID 9653641 and are consistent with what has been descirbed in PMID 15077190.

    • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

      Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

      A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

      Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

      Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

       

       

    • OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)
      Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (15)

    Publishing research using ab92514? Please let us know so that we can cite the reference in this datasheet.

    ab92514 has been referenced in 15 publications.

    • Ben Kacem M  et al. Variation of 4 MV X-ray dose rate strongly impacts biological response both in vitro and in vivo. Sci Rep 10:7021 (2020). PubMed: 32341396
    • Raghu D  et al. GALNT3 Maintains the Epithelial State in Trophoblast Stem Cells. Cell Rep 26:3684-3697.e7 (2019). PubMed: 30917321
    • Ding X  et al. MicroRNA-143-3p suppresses tumorigenesis by targeting catenin-d1 in colorectal cancer. Onco Targets Ther 12:3255-3265 (2019). PubMed: 31118676
    • Xu S  et al. Discoidin domain receptor 1 deficiency in vascular smooth muscle cells leads to mislocalisation of N-cadherin contacts. Biol Open 8:N/A (2019). PubMed: 31362952
    • An Y  et al. Cdh1 and Pik3ca Mutations Cooperate to Induce Immune-Related Invasive Lobular Carcinoma of the Breast. Cell Rep 25:702-714.e6 (2018). PubMed: 30332649
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-delta 1 Catenin antibody [EPR357(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Undifferentiated Human ES cells on MEFs)
    Permeabilization
    Yes - see blocking buffer
    Specification
    Undifferentiated Human ES cells on MEFs
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-delta 1 Catenin antibody [EPR357(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEPG2)
    Specification
    HEPG2
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Rebecca Atkinson-Dell

    Verified customer

    Submitted Nov 18 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-delta 1 Catenin antibody [EPR357(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Sample
    Mouse Cell (mouse embryonic stem cell)
    Specification
    mouse embryonic stem cell
    Permeabilization
    Yes - 0.1% triton x
    Fixative
    methanol:acetone 1:1
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

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