Recombinant
RabMAb

Recombinant Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 950-1050. The exact sequence is proprietary.

  • General notes

    Ab239916 is the carrier-free version of ab92514. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239916 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.

Target

  • Function

    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain

    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications

    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • delta 1 Catenin antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

References

ab239916 has not yet been referenced specifically in any publications.

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