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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins
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RecombinantRabMAb

Recombinant Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

  • Datasheet
Reviews (1)Q&A (1)

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Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
  • Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
  • Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
  • Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [YE372]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for human delta 1 Catenin/CAS (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin/CAS.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 50-150 (N terminal). The exact sequence is proprietary.

  • Epitope

    ab32095 reacts with an epitope located in the N terminal region of delta 1 Catenin
  • Positive control

    • HeLa whole cell lysate (ab150035). human breast carcinoma
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    YE372
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-delta 1 Catenin/CAS antibody [YE372] (ab202913)
    • HRP Anti-delta 1 Catenin/CAS antibody [YE372] (ab202914)
    • Alexa Fluor® 647 Anti-delta 1 Catenin/CAS antibody [YE372] (ab203148)
    • Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (ab247228)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)

Applications

Our Abpromise guarantee covers the use of ab32095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 108 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function

    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain

    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications

    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Target information above from: UniProt accession O60716 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1500 Human
    • Omim: 601045 Human
    • SwissProt: O60716 Human
    • Unigene: 166011 Human
    • Alternative names

      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Images

    • Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095) at 1/5000 dilution + Hela cell lysate.

      Predicted band size: 108 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

      ab32095 at a 1/100 dilution staining delta 1 Catenin/CAS in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab32095 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
    • Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

      Purified ab32095 at 1/50 dilution (2µg) immunoprecipitating delta 1 Catenin/CAS in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab32095 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32095 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 120 kDa
      Multi-bands could be isoforms of delta 1 Catenin based on Uniprot.

    • Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)
      Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab32095? Please let us know so that we can cite the reference in this datasheet.

    ab32095 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Inquiry: Kindly provide details about Dilution, antigen retrieval and PH for P120. Its my research antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2015

    Answer

    I have contacted the laboratory who tested the protocol, and they have kindly provided the following general protocol which has been used. The antibody was tested some time ago but they probably used 0.01M Sodium Citrate Buffer (pH 6.0 antigen retrieval buffer at that time. Please note that the protocol including the antibody dilution and suggested antigen retrieval would be a guideline only and may require some further optimization. For antibody dilution, we usually suggest a titration experiment using different dilutions to help optimize (e.g start with 1:100, 1:500, 1:1000).

    I hope this will be helpful. Please do not hesitate to contact me if you require any further information.

    Protocol:

    1. Solutions and reagents
    1.1. Xylene 1.2. Ethanol, anhydrous denatured, histological grade (100%, 95%, 70%, 50%) 1.3. Washing buffer/TBST: 1X TBS/0.1% Tween-20, pH to 7.6. 1.4. Distilled water (dH2O)
    1.5. Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0
    To prepare Antigen Retrieval stock solutions:
    10X Stock: Dissolve 29.4 g sodium citrate trisodium salt dehydrate
    (C 6H 5Na 3O 7 ·2H2O in 1 liter of dH2O. Add 5mL Tween-20. 1X Working Solution: Mix 200mL 10X stock with 1800mL dH2O; pH to 6.0
    1.6. 3% Hydrogen Peroxide 1.7. Blocking Buffer: 10% serum in PBS (serum origin depends on the host of the secondary antibody)
    1.8. Primary Antibody Diluent: 5% serum in PBS (serum origin depends on the host of the
    Secondary antibody)
    1.9. Hematoxylin 1.10. Permanent Mounting Medium

    2. Protocol
    2.1. Deparaffinization/Rehydration
    2.1.1. Heat slides in an oven at 65oC for 1 hour.
    2.1.2. De-paraffinize/hydrate using the following series of washes: two Xylene washes (3 min each), followed by two 100% ethanol rinses (3 min each), followed by 95% ethanol, 70% ethanol, 50% ethanol, 30% ethanol, followed by TBST wash for 3 min on a shaker.
    2.2. Antigen Retrieval
    This is recommended Heat Induced Epitope Retrieval (HIER) using Decloaking
    Chamber/Pressure Cooker. Hot water bath or Microwave with temperature sensor can be also used
    (protocol would vary depending on the method used).
    2.2.1. Add 500 ml of dH 2O to Decloaker/Pressure Cooker.
    2.2.2. Immerse slides into staining dish containing Antigen Retrieval Solution. Place stainingdish into decloaking chamber.
    2.2.3. Program to run for 30 seconds at 125oC, followed by 10 seconds at 90oC.
    2.2.4. Let it cool down to room temperature (10 – 20 minutes).
    2.2.5. Removes slides and rinse in TBST. 2.2.6. Proceed to Staining step.
    2.3. Staining
    2.3.1. Wash slides with TBST for 3 min on a shaker.
    2.3.2. Inactivate endogenous peroxidase by covering tissue with 3% hydrogen peroxide for 5 min.
    2.3.3. Wash slides three times with TBST (3 min each on a shaker).
    2.3.4. Block slides with the blocking solution for 1 hour.
    2.3.5. Dilute primary antibody in primary antibody diluent per recommendation on data sheet.
    2.3.6. Apply primary antibody to each section and incubate overnight in the humidified chamber (4oC).
    2.3.7. Wash slides three times with TBST (3 min each on a shaker).
    2.3.8. Apply to each section secondary HRP-conjugated anti-rabbit antibody diluted in the blocking solution per manufacturer’s recommendation; incubate for 30 min at room temperature.
    2.3.9. Wash slides three times with TBST (5 min each on a shaker).
    2.3.10. Add freshly prepared DAB substrate to the sections and incubate until stain develops (generally 1 min).
    2.3.11. Rinse sections with water.
    2.3.12. Counterstain with Hematoxylin (generally 10 seconds).
    2.3.13. Rinse sections with water.
    2.3.14. Dehydrate samples using two washes with 100% Ethanol (3 min each), followed by
    two rinses with Xylene (3 min each).
    2.3.15. Mount on slides using permanent mounting medium.

    Read More

    Sam Washer

    Abcam Scientific Support

    Answered on Sep 18 2015

    Western blot abreview for Anti-delta 1 Catenin/CAS antibody [YE372]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (platelets)
    Loading amount
    15 µg
    Treatment
    various agonists for 3 minutes
    Specification
    platelets
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 13 2006

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