Overview

  • Product name
    Anti-delta 1 Catenin/CAS antibody [YE372]
    See all delta 1 Catenin/CAS primary antibodies
  • Description
    Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for human delta 1 Catenin (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 50-150 (N terminal). The exact sequence is proprietary.

  • Epitope
    ab32095 reacts with an epitope located in the N terminal region of delta 1 Catenin
  • Positive control
    • Hela cell lysate. human breast carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 108 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/80.

Target

  • Function
    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity
    Expressed in vascular endothelium.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain
    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications
    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization
    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • delta 1 Catenin antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Images

  • Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095) at 1/5000 dilution + Hela cell lysate.

    Predicted band size: 108 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

  • ab32095 at a 1/100 dilution staining delta 1 Catenin in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

References

ab32095 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I have contacted the laboratory who tested the protocol, and they have kindly provided the following general protocol which has been used. The antibody was tested some time ago but they probably used 0.01M Sodium Citrate Buffer (pH 6.0 antigen retrieval buffer at that time. Please note that the protocol including the antibody dilution and suggested antigen retrieval would be a guideline only and may require some further optimization. For antibody dilution, we usually suggest a titration experiment using different dilutions to help optimize (e.g start with 1:100, 1:500, 1:1000).

I hope this will be helpful. Please do not hesitate to contact me if you require any further information.

Protocol:

1. Solutions and reagents
1.1. Xylene 1.2. Ethanol, anhydrous denatured, histological grade (100%, 95%, 70%, 50%) 1.3. Washing buffer/TBST: 1X TBS/0.1% Tween-20, pH to 7.6. 1.4. Distilled water (dH2O)
1.5. Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0
To prepare Antigen Retrieval stock solutions:
10X Stock: Dissolve 29.4 g sodium citrate trisodium salt dehydrate
(C 6H 5Na 3O 7 ·2H2O in 1 liter of dH2O. Add 5mL Tween-20. 1X Working Solution: Mix 200mL 10X stock with 1800mL dH2O; pH to 6.0
1.6. 3% Hydrogen Peroxide 1.7. Blocking Buffer: 10% serum in PBS (serum origin depends on the host of the secondary antibody)
1.8. Primary Antibody Diluent: 5% serum in PBS (serum origin depends on the host of the
Secondary antibody)
1.9. Hematoxylin 1.10. Permanent Mounting Medium

2. Protocol
2.1. Deparaffinization/Rehydration
2.1.1. Heat slides in an oven at 65oC for 1 hour.
2.1.2. De-paraffinize/hydrate using the following series of washes: two Xylene washes (3 min each), followed by two 100% ethanol rinses (3 min each), followed by 95% ethanol, 70% ethanol, 50% ethanol, 30% ethanol, followed by TBST wash for 3 min on a shaker.
2.2. Antigen Retrieval
This is recommended Heat Induced Epitope Retrieval (HIER) using Decloaking
Chamber/Pressure Cooker. Hot water bath or Microwave with temperature sensor can be also used
(protocol would vary depending on the method used).
2.2.1. Add 500 ml of dH 2O to Decloaker/Pressure Cooker.
2.2.2. Immerse slides into staining dish containing Antigen Retrieval Solution. Place stainingdish into decloaking chamber.
2.2.3. Program to run for 30 seconds at 125oC, followed by 10 seconds at 90oC.
2.2.4. Let it cool down to room temperature (10 – 20 minutes).
2.2.5. Removes slides and rinse in TBST. 2.2.6. Proceed to Staining step.
2.3. Staining
2.3.1. Wash slides with TBST for 3 min on a shaker.
2.3.2. Inactivate endogenous peroxidase by covering tissue with 3% hydrogen peroxide for 5 min.
2.3.3. Wash slides three times with TBST (3 min each on a shaker).
2.3.4. Block slides with the blocking solution for 1 hour.
2.3.5. Dilute primary antibody in primary antibody diluent per recommendation on data sheet.
2.3.6. Apply primary antibody to each section and incubate overnight in the humidified chamber (4oC).
2.3.7. Wash slides three times with TBST (3 min each on a shaker).
2.3.8. Apply to each section secondary HRP-conjugated anti-rabbit antibody diluted in the blocking solution per manufacturer’s recommendation; incubate for 30 min at room temperature.
2.3.9. Wash slides three times with TBST (5 min each on a shaker).
2.3.10. Add freshly prepared DAB substrate to the sections and incubate until stain develops (generally 1 min).
2.3.11. Rinse sections with water.
2.3.12. Counterstain with Hematoxylin (generally 10 seconds).
2.3.13. Rinse sections with water.
2.3.14. Dehydrate samples using two washes with 100% Ethanol (3 min each), followed by
two rinses with Xylene (3 min each).
2.3.15. Mount on slides using permanent mounting medium.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (platelets)
Loading amount
15 µg
Treatment
various agonists for 3 minutes
Specification
platelets
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

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Verified customer

Submitted Dec 13 2006

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