Recombinant
RabMAb

Recombinant Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [YE372]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for human delta 1 Catenin/CAS (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin/CAS.

  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 50-150 (N terminal). The exact sequence is proprietary.

  • Epitope

    ab32095 reacts with an epitope located in the N terminal region of delta 1 Catenin
  • Positive control

    • HeLa whole cell lysate (ab150035). human breast carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 108 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/80.

Target

  • Function

    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain

    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications

    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • delta 1 Catenin antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Images

  • Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095) at 1/5000 dilution + Hela cell lysate.

    Predicted band size: 108 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

  • ab32095 at a 1/100 dilution staining delta 1 Catenin/CAS in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab32095 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (platelets)
Loading amount
15 µg
Treatment
various agonists for 3 minutes
Specification
platelets
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Dec 13 2006

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