Recombinant
RabMAb

Recombinant Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318)

Overview

  • Product name

    Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310)
  • Host species

    Rabbit
  • Specificity

    The antibody only detects delta 1 Catenin/CAS when phosphorylated on Threonine 310.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS (phospho T310). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysates treated with calyculin A Human breast carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 108 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificity

      Expressed in vascular endothelium.
    • Sequence similarities

      Belongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • Domain

      A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications

      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localization

      Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Images

    • All lanes : Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318) at 1/5000 dilution

      Lane 1 : 293T cell lysates untreated
      Lane 2 : 293T cell lysates treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 108 kDa
      Observed band size: 108 kDa
      Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab81318 at 1/100 dilution.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    ab81318 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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