Product nameAnti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382]
See all delta 1 Catenin/CAS primary antibodies
DescriptionRabbit monoclonal [EPR2382] to delta 1 Catenin/CAS (phospho T310)
The antibody only detects delta 1 Catenin/CAS when phosphorylated on Threonine 310.
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human delta 1 Catenin/CAS (phospho T310). The exact sequence is proprietary.
- 293T cell lysates treated with calyculin A Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab81318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 108 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family.
Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
All lanes : Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318) at 1/5000 dilution
Lane 1 : 293T cell lysates untreated
Lane 2 : 293T cell lysates treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab81318 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab81318 has not yet been referenced specifically in any publications.