Product nameAnti-delta 1 Catenin/CAS (phospho Y228) antibody [E205]
See all delta 1 Catenin/CAS primary antibodies
DescriptionRabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
SpecificityThis antibody only detects delta 1 Catenin phosphorylated on Tyrosine 228.
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y228).
- WB: HeLa cell lysate. IHC-P: Breast carcinoma.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).|
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family.
Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
All lanes : Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
Lane 1 : HeLa cell lysate, untreated.
Lane 2 : HeLa cell lysate, treated with Pervanandate.
Lane 3 : HeLa cell lysate, treated with Phosphatase.
Predicted band size: 108 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Ab32403, at a 1/50 dilution, staining delta 1 Catenin in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.
This product has been referenced in:
- Park SY et al. Glycoprotein 90K Promotes E-Cadherin Degradation in a Cell Density-Dependent Manner via Dissociation of E-Cadherin-p120-Catenin Complex. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29207493) »
- Surana R et al. IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model. Cancer Immunol Res 2:1103-12 (2014). WB . Read more (PubMed: 25204776) »