Recombinant
RabMAb

Recombinant Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] - BSA and Azide free (ab247254)

Overview

  • Product name

    Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] - BSA and Azide free
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects delta 1 Catenin/CAS phosphorylated on Tyrosine 228. 

  • Tested applications

    Suitable for: ICC, IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS (phospho Y228). The exact sequence is proprietary.

  • General notes

    Ab247254 is the carrier-free version of ab32403. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247254 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificity

      Expressed in vascular endothelium.
    • Sequence similarities

      Belongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • Domain

      A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications

      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localization

      Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    References

    ab247254 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab247254.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up