Recombinant
RabMAb

Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578)

Overview

  • Product name
    Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)]
    See all delta 1 Catenin/CAS primary antibodies
  • Description
    Rabbit monoclonal [EPR2384(2)] to delta 1 Catenin/CAS (phospho Y280)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human delta 1 Catenin/CAS aa 250-350 (phospho Y280) (Cysteine residue). The exact sequence is proprietary.
    Database link: O60716

  • Positive control
    • Pervanadate treated A431 cell lysate, Human gastric adenocarcinoma
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab173578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/20000. Predicted molecular weight: 90-120 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificity
      Expressed in vascular endothelium.
    • Sequence similarities
      Belongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • Domain
      A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications
      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localization
      Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • delta 1 Catenin antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from A431 cells treated with pervanadate lysate: ab173578 at 1/10 dilution immunoprecipitated delta 1 Catenin (phospho Y280).

    • Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling  delta 1 Catenin (phospho Y280) with ab173578 at 1/50 dilution

    • All lanes : Anti-delta 1 Catenin/CAS (phospho Y280) antibody [EPR2384(2)] (ab173578) at 1/50000 dilution

      Lane 1 : Untreated A431 cell lysate
      Lane 2 : Pervanadate treated A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 90-120 kDa

    References

    ab173578 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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