Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)
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- References
- Protocols
Overview
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Product name
Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [EP2385Y] to delta 1 Catenin/CAS (phospho Y291) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y291).
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Positive control
- Jurkat cell lysate treated with pervanadate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2385Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab81274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/20000. Predicted molecular weight: 108 kDa. |
Target
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Function
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
Domain
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
Post-translational
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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Alternative names
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
Images
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![Western blot - Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)](https://www.abcam.com/ps/products/81/ab81274/Images/ab81274-96456-anti-delta-1-catenin-cas-phospho-y291-antibody-ep2385y-western-blot.jpg)
Western blot - Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274)All lanes : Anti-delta 1 Catenin/CAS (phospho Y291) antibody [EP2385Y] (ab81274) at 1/20000 dilution
Lane 1 : Jurkat cell lysate untreated
Lane 2 : Jurkat cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Protocols
Datasheets and documents
References
ab81274 has not yet been referenced specifically in any publications.
