Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2386] to delta 1 Catenin/CAS (phospho Y96)
- Suitable for: ICC, WB
- Reacts with: Human
Product nameAnti-delta 1 Catenin/CAS (phospho Y96) antibody [EPR2386]
See all delta 1 Catenin/CAS primary antibodies
DescriptionRabbit monoclonal [EPR2386] to delta 1 Catenin/CAS (phospho Y96)
ab75772 only detects delta 1 Catenin/CAS phosphorylated on Tyrosine 96.
Tested applicationsSuitable for: ICC, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y96).
- A431 cell lysates, untreated or treated with pervanadate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/250.|
|WB||1/25000 - 1/50000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).|
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family.
Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
All lanes : Anti-delta 1 Catenin/CAS (phospho Y96) antibody [EPR2386] (ab75772) at 1/50000 dilution
Lane 1 : A431 cell lysates, untreated
Lane 2 : A431 cell lysates, treated with
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
ab75772 has not yet been referenced specifically in any publications.