Anti-delta Sarcoglycan antibody (ab115019)
Key features and details
- Goat polyclonal to delta Sarcoglycan
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-delta Sarcoglycan antibody
See all delta Sarcoglycan primary antibodies -
Description
Goat polyclonal to delta Sarcoglycan -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
C-RLPHGSYTPTGTR
, corresponding to internal sequence amino acids 245-257 of Human delta Sarcoglycan(NP_000328.2; NP_758447.1; NP_001121681.1). -
Positive control
- Human and Rat heart lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab115019 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. -
Tissue specificity
Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung. -
Involvement in disease
Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the sarcoglycan beta/delta/gamma/zeta family. -
Post-translational
modificationsGlycosylated.
Disulfide bonds are present. -
Cellular localization
Cell membrane > sarcolemma. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 6444 Human
- Entrez Gene: 24052 Mouse
- Omim: 601411 Human
- SwissProt: Q92629 Human
- SwissProt: P82347 Mouse
- Unigene: 387207 Human
- Unigene: 644733 Human
- Unigene: 338890 Mouse
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Alternative names
- 35 kDa dystrophin associated glycoprotein antibody
- 35 kDa dystrophin-associated glycoprotein antibody
- 35DAG antibody
see all
Images
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Anti-delta Sarcoglycan antibody (ab115019) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab115019 has not yet been referenced specifically in any publications.