Overview

  • Product name

    Anti-Delta-catenin antibody [EPR17628]
    See all Delta-catenin primary antibodies
  • Description

    Rabbit monoclonal [EPR17628] to Delta-catenin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Delta-catenin aa 1-100. The exact sequence is proprietary.
    Database link: O35927

  • Positive control

    • WB: Mouse brain, hippocampus and cerebellum; and rat brain and hippocampus tissue lysates. PC-12 whole cell lysate. ICC/IF: Neuro-2a cells.
  • General notes

     

     

     This product was previously labelled as delta 2 Catenin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).
ICC/IF 1/500.

Target

  • Function

    Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules.
  • Tissue specificity

    Predominantly expressed in brain.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • Post-translational
    modifications

    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cell junction > adherens junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARM-repeat protein antibody
    • Catenin (cadherin associated protein) delta 2 antibody
    • catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody
    • Catenin delta 2 antibody
    • Catenin delta-2 antibody
    • CTND2_HUMAN antibody
    • CTNND 2 antibody
    • Ctnnd2 antibody
    • Delta catenin antibody
    • Delta-catenin antibody
    • GT 24 antibody
    • GT24 antibody
    • Neural plakophilin related arm repeat protein antibody
    • Neural plakophilin related armadillo repeat protein antibody
    • Neural plakophilin-related ARM-repeat protein antibody
    • Neural plakophilin-related, Neurojungin antibody
    • Neurojungin antibody
    • NPRAP antibody
    • T cell delta catenin antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

  • All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 10 seconds

  • All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Rat hippocampus lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 3 minutes

  • All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 10 seconds

References

ab184917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184917.
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