Anti-Delta Opioid Receptor antibody (ab10272)

Reviews (1)Q&A (3)References (1)

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: Whole serum

  • Purity

    Whole antiserum

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.

    • Target

    • Relevance

      Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.

    • Cellular localization

      Multi pass membrane protein

    • Database links

      see all

    • Alternative names

      • D-OR-1 antibody
      • Delta type opioid receptor antibody
      • Delta type opioid receptor DOR 1 antibody
      • Delta type opioid receptor DOR1 antibody
      • Delta-type opioid receptor antibody
      • DOR 1 antibody
      • DOR-1 antibody
      • DOR1 antibody
      • mDOR antibody
      • Nbor antibody
      • Opioid receptor delta 1 antibody
      • OPRD 1 antibody
      • OPRD antibody
      • OPRD_HUMAN antibody
      • Oprd1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-Delta Opioid Receptor antibody (ab10272)
      Immunocytochemistry - Anti-Delta Opioid Receptor antibody (ab10272)
      ab10272 at 1/1000 dilution staining Delta Opioid Receptor in transfected cells by Immunocytochemistry.

    References

    This product has been referenced in:

    • Koizumi H  et al. Response of striosomal opioid signaling to dopamine depletion in 6-hydroxydopamine-lesioned rat model of Parkinson's disease: a potential compensatory role. Front Cell Neurosci 7:74 (2013). Read more (PubMed: 23730270) »
    See 1 Publication for this product

    Publishing research using ab10272? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Delta Opioid Receptor antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Human Neuroblastoma SHSY5Y)
    Specification
    Human Neuroblastoma SHSY5Y
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Mrs. Nada Charbaji

    Verified customer

    Submitted Sep 20 2010

    Question

    Please can you let me know about the molecular weight of the protein it detects,as i want to go for western bloting

    Read More
    Answer

    Thank you for your enquiry. Unfortunately, this product has not been tested in western blot application. However, by running a SwissProt analysis, the expected molecular weight is around 40kDa. I hope this information will be useful to you. Should you require any further information, please do not hesitate to contact me.

    Read More

    Question

    We have recently tried one of your anti-delta opioid receptor antibodies (ab10272). It worked in IF in the cells expressing human receptor, however there was some background seen in non-expressing cells. I wonder if you are planning to do affinity purification of this antibody. If not than we would be interested in buying 300-400 microliters. Can you give us discount for that? Lastly, I would like to bring it to your attention that there is a mistake in the datasheet for this product, the region the antibody was raised against is 360-372 not 122-138.

    Read More
    Answer

    Mark forwarded your request for a discount on ab10272 to my attention. We sell this product in units of 50µl and you have advised that you are likely to purchase 300-400 µl or 6-8 units. I would be pleased to offer you a 15% discount if you purchase 6-8 units on the same purchase order. Please quote reference DMMF1905-15% when placing your order to ensure that the appropriate discount is deducted.

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    Question

    We already have several delta opioid receptor antibodies from different companies. However, we did not have much luck with them. We use CHO cells expressing human DOR1 to characterize antibodies in Western blots and cell-based assay that is most similar to ICC. Two antibodies from Santa Cruz, and one from USB detected band of about 60 kDa on WB, however the same band was seen in CHO cells expressing kappa receptor or non-expressing CHOK1 cells. Biosourse antibody recognized 45 kDa band in a similar manner, and Chemicon's antibody just stained everything. The only antibody that works nicely is phosphospecific antibody to (P)Ser363 from CST. It detects delta agonist-dependent phosphorylation at 60 kDA band in CHOhDOR cells only. The results of cell-based assay agree with the WB for all antibodies tested. I would be most grateful if you could give me technical advice. Do you compare your antibodies to the ones from competitors?

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. We would like to confirm that ab10272 has only been tested and characterised for ICC application, and we do not have any information if it works for Western. We are very sorry to inform you that do not compare our antibodies to the ones from competitors. However, Abcam policy dictates that if a product does not performed as stated on the datasheet, we will offer a full reimbursement or provide a replacement of equivalent value.

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