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We already have several delta opioid receptor antibodies from different companies. However, we did not have much luck with them. We use CHO cells expressing human DOR1 to characterize antibodies in Western blots and cell-based assay that is most similar to ICC. Two antibodies from Santa Cruz, and one from USB detected band of about 60 kDa on WB, however the same band was seen in CHO cells expressing kappa receptor or non-expressing CHOK1 cells. Biosourse antibody recognized 45 kDa band in a similar manner, and Chemicon's antibody just stained everything. The only antibody that works nicely is phosphospecific antibody to (P)Ser363 from CST. It detects delta agonist-dependent phosphorylation at 60 kDA band in CHOhDOR cells only. The results of cell-based assay agree with the WB for all antibodies tested. I would be most grateful if you could give me technical advice. Do you compare your antibodies to the ones from competitors?
Asked on Mar 16 2004
Thank you for your enquiry and your interest in our products. We would like to confirm that ab10272 has only been tested and characterised for ICC application, and we do not have any information if it works for Western. We are very sorry to inform you that do not compare our antibodies to the ones from competitors. However, Abcam policy dictates that if a product does not performed as stated on the datasheet, we will offer a full reimbursement or provide a replacement of equivalent value.
Answered on Mar 17 2004