Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Delta Opioid Receptor antibody [EPR5029(2)]
See all Delta Opioid Receptor primary antibodies
DescriptionRabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
Tested applicationsSuitable for: ICC, WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P41143
- WB: Human brain, Mouse spleen, Rat spleen, and Mouse brain lysates\t\t\tICC/IF: SH-SY5Y cells. Flow Cyt: U-87 MG cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
|Flow Cyt||1/10 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceFunction: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.
Cellular localizationMulti pass membrane protein
- D-OR-1 antibody
- Delta type opioid receptor antibody
- Delta type opioid receptor DOR 1 antibody
All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/2000 dilution (Purified)
Lane 1 : Human brain lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/10000 dilution (Purified) + Mouse brain lysates at 20 µg
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : HUVEC lysate
Lane 5 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor®488 Goat anti-Rabbit secondary IgG (1/1000).
Flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red) or a rabbit IgG control (green).
ab176324 has been referenced in 6 publications.
- Blackwood CA et al. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Mol Neurobiol 56:3603-3615 (2019). PubMed: 30155791
- Zhang K et al. Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway. Int Immunopharmacol 73:23-40 (2019). PubMed: 31078923
- Blackwood CA et al. Escalated Oxycodone Self-Administration and Punishment: Differential Expression of Opioid Receptors and Immediate Early Genes in the Rat Dorsal Striatum and Prefrontal Cortex. Front Neurosci 13:1392 (2019). PubMed: 31998063
- Suo J et al. Met-enkephalin improves metabolic syndrome in high fat diet challenged mice through promotion of adipose tissue browning. Toxicol Appl Pharmacol 359:12-23 (2018). PubMed: 30222981
- Rice FL et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962
- Leoncikas V et al. Generation of 2,000 breast cancer metabolic landscapes reveals a poor prognosis group with active serotonin production. Sci Rep 6:19771 (2016). PubMed: 26813959