Recombinant
RabMAb

Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Overview

  • Product name
    Anti-Delta Opioid Receptor antibody [EPR5029(2)]
    See all Delta Opioid Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P41143

  • Positive control
    • Human cerebellum, fetal brain, U87-MG, HUVEC and SH-SY5Y lysates. U87-MG cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR5029(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance
      Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.
    • Cellular localization
      Multi pass membrane protein
    • Database links
    • Alternative names
      • D-OR-1 antibody
      • Delta type opioid receptor antibody
      • Delta type opioid receptor DOR 1 antibody
      • Delta type opioid receptor DOR1 antibody
      • Delta-type opioid receptor antibody
      • DOR 1 antibody
      • DOR-1 antibody
      • DOR1 antibody
      • mDOR antibody
      • Nbor antibody
      • Opioid receptor delta 1 antibody
      • OPRD 1 antibody
      • OPRD antibody
      • OPRD_HUMAN antibody
      • Oprd1 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

       

      Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor®488 Goat anti-Rabbit secondary IgG (1/1000).

    • All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : U87-MG lysate
      Lane 4 : HUVEC lysate
      Lane 5 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red) or a rabbit IgG control (green).

    References

    This product has been referenced in:
    • Leoncikas V  et al. Generation of 2,000 breast cancer metabolic landscapes reveals a poor prognosis group with active serotonin production. Sci Rep 6:19771 (2016). Read more (PubMed: 26813959) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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