Product nameAnti-delta Sarcoglycan antibody [EPR8706]
See all delta Sarcoglycan primary antibodies
DescriptionRabbit monoclonal [EPR8706] to delta Sarcoglycan
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
Synthetic peptide within Human delta Sarcoglycan aa 200-300. The exact sequence is proprietary.
- Human fetal heart and skeletal muscle lysates; Human heart tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Tissue specificityMost strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Involvement in diseaseDefects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the sarcoglycan beta/delta/gamma/zeta family.
Disulfide bonds are present.
Cellular localizationCell membrane > sarcolemma. Cytoplasm > cytoskeleton.
- Information by UniProt
- 35 kDa dystrophin associated glycoprotein antibody
- 35 kDa dystrophin-associated glycoprotein antibody
- 35DAG antibody
Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling delta Sarcoglycan with ab137101 at 1/100 dilution.
All lanes : Anti-delta Sarcoglycan antibody [EPR8706] (ab137101) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Boyer JG et al. ERK1/2 signaling induces skeletal muscle slow fiber-type switching and reduces muscular dystrophy disease severity. JCI Insight 5:N/A (2019). Read more (PubMed: 30964448) »
- Vanhoutte D et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB . Read more (PubMed: 27669143) »