Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8706] to delta Sarcoglycan
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-delta Sarcoglycan antibody [EPR8706]
See all delta Sarcoglycan primary antibodies
DescriptionRabbit monoclonal [EPR8706] to delta Sarcoglycan
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
Synthetic peptide within Human delta Sarcoglycan aa 200-300. The exact sequence is proprietary.
- Human fetal heart and skeletal muscle lysates; Human heart tissue. IP: Human skeletal muscle lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Tissue specificityMost strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Involvement in diseaseDefects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the sarcoglycan beta/delta/gamma/zeta family.
Disulfide bonds are present.
Cellular localizationCell membrane > sarcolemma. Cytoplasm > cytoskeleton.
- Information by UniProt
- 35 kDa dystrophin associated glycoprotein antibody
- 35 kDa dystrophin-associated glycoprotein antibody
- 35DAG antibody
Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling delta Sarcoglycan with ab137101 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-delta Sarcoglycan antibody [EPR8706] (ab137101) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Purified ab137101 at 1/20 dilution (1µg) immunoprecipitating delta Sarcoglycan in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle lysate 10µg
Lane 2 (+): ab137101 + Human skeletal muscle lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137101 in Human skeletal muscle lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 35 kDa
ab137101 has been referenced in 3 publications.
- Boyer JG et al. ERK1/2 signaling induces skeletal muscle slow fiber-type switching and reduces muscular dystrophy disease severity. JCI Insight 5:N/A (2019). PubMed: 30964448
- Vanhoutte D et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB . PubMed: 27669143
- Tjondrokoesoemo A et al. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet 25:1192-202 (2016). PubMed: 26744329