Overview

  • Product name

    Anti-delta Sarcoglycan antibody [EPR8706]
    See all delta Sarcoglycan primary antibodies
  • Description

    Rabbit monoclonal [EPR8706] to delta Sarcoglycan
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human delta Sarcoglycan aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart and skeletal muscle lysates; Human heart tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
    • Tissue specificity

      Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
    • Involvement in disease

      Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
      Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the sarcoglycan beta/delta/gamma/zeta family.
    • Post-translational
      modifications

      Glycosylated.
      Disulfide bonds are present.
    • Cellular localization

      Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • 35 kDa dystrophin associated glycoprotein antibody
      • 35 kDa dystrophin-associated glycoprotein antibody
      • 35DAG antibody
      • CMD1L antibody
      • DAGD antibody
      • Delta-sarcoglycan antibody
      • Delta-SG antibody
      • Dystrophin associated glycoprotein delta sarcoglycan antibody
      • LGMD2F antibody
      • MGC22567 antibody
      • Placental delta sarcoglycan antibody
      • Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) antibody
      • SG delta antibody
      • SGCD antibody
      • SGCD_HUMAN antibody
      • SGCDP antibody
      • SGD antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling delta Sarcoglycan with ab137101 at 1/100 dilution.
    • All lanes : Anti-delta Sarcoglycan antibody [EPR8706] (ab137101) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 32 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Boyer JG  et al. ERK1/2 signaling induces skeletal muscle slow fiber-type switching and reduces muscular dystrophy disease severity. JCI Insight 5:N/A (2019). Read more (PubMed: 30964448) »
    • Vanhoutte D  et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB . Read more (PubMed: 27669143) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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