Overview

  • Product name
    Anti-DeltaD antibody [Dld 5C7/2, zdd2]
    See all DeltaD primary antibodies
  • Description
    Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD
  • Host species
    Mouse
  • Specificity
    This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, Flow Cyt, ICC/IF, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Extracellular DeltaD peptide (Zebrafish).

  • Epitope
    Zebrafish DeltaD protein extracellular domain.
  • Positive control
    • HEK293 cells transfected with deltaD or zebrafish embryo.

Properties

Applications

Our Abpromise guarantee covers the use of ab73331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.

Target

  • Relevance
    DeltaD is one of the 4 members of the zebrafish Delta family of Notch ligands, important in the control of cell differentiation in many tissues. DeltaD acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. It can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized.
  • Cellular localization
    Cell Membrane
  • Database links
    • Alternative names
      • Aei antibody
      • After eight antibody
      • Dld antibody

    Images

    • ab73331 staining DeltaD in wild-type (upper panel) and ktoy82 (lower panel) zebrafish embryo hindbrain, by Immunohistochemistry - Wholemount.

    References

    This product has been referenced in:
    • Gallagher TL  et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). Read more (PubMed: 28648842) »
    • Rothschild SC  et al. CaMK-II activation is essential for zebrafish inner ear development and acts through Delta-Notch signaling. Dev Biol 381:179-88 (2013). Read more (PubMed: 23747599) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Application
    IHC - Wholemount
    Sample
    Zebrafish Tissue sections (whole embryo)
    Specification
    whole embryo

    Ms. Emerald Butko

    Verified customer

    Submitted Oct 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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