IHC - Wholemount abreview for Anti-DeltaD antibody [Dld 5C7/2, zdd2]

Good
Abreviews
Application
IHC - Wholemount
Sample
Zebrafish Tissue sections (whole embryo)
Specification
whole embryo

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: maleic acid blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 594 donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
Whole-mount ISH on 18-somite stage zebrafish embryos fixed in 4%PFA.

Staining observed in somitic tissue.

Maleic acid blocking solution is: 150mM maleic acid, 100mM NaCl, 2% blocking reagent (roche), pH7.5, 0.1% Tween-20

Ms. Emerald Butko

Verified customer

Submitted Oct 24 2011

Sign up