Product nameAnti-Dematin antibody
See all Dematin primary antibodies
DescriptionRabbit polyclonal to Dematin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Synthetic peptide within Human Dematin aa 225-275. The exact sequence is proprietary.
Database link: Q08495
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226357 was affinity purified using an epitope specific to Dematin immobilized on solid support.
Our Abpromise guarantee covers the use of ab226357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 46 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActin-bundling protein. May function in mitogen-activated protein kinase pathway.
Tissue specificityHeart, brain, lung, skeletal muscle, and kidney.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 1 HP (headpiece) domain.
DomainContains at least two actin-binding sites, one in the headpiece domain and one in the amino-terminal portion.
Consists of a large core fragment, the amino-terminal portion, and a small headpiece, the C-terminal portion. The headpiece can bind but cannot bundle actin filaments.
modificationsActin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.
The N-terminus is blocked.
- Information by UniProt
- DEMA antibody
- DEMA_HUMAN antibody
- Dematin antibody
All lanes : Anti-Dematin antibody (ab226357) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 75 seconds
Cell lysates were prepared using NETN lysis buffer.
Dematin was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226357 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226357 at 1 µg/ml.
Lane 1: ab226357 IP in HepG2 whole cell lysate.
Lane 2: Control IgG IP in HepG2 whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226357 has not yet been referenced specifically in any publications.