Overview

  • Product name

  • Description

    Rabbit polyclonal to Dematin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human Dematin aa 225-275. The exact sequence is proprietary.
    Database link: Q08495

  • Positive control

    • WB: HEK-293T and HepG2 whole cell lysate (ab7900). IP: HepG2 whole cell lysate (ab7900).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226357 was affinity purified using an epitope specific to Dematin immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 46 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Actin-bundling protein. May function in mitogen-activated protein kinase pathway.
  • Tissue specificity

    Heart, brain, lung, skeletal muscle, and kidney.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 1 HP (headpiece) domain.
  • Domain

    Contains at least two actin-binding sites, one in the headpiece domain and one in the amino-terminal portion.
    Consists of a large core fragment, the amino-terminal portion, and a small headpiece, the C-terminal portion. The headpiece can bind but cannot bundle actin filaments.
  • Post-translational
    modifications

    Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.
    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DEMA antibody
    • DEMA_HUMAN antibody
    • Dematin antibody
    • DMT antibody
    • EPB49 antibody
    • erythrocyte membrane protein band 4.9 (dematin) antibody
    • Erythrocyte membrane protein band 4.9 antibody
    see all

Images

  • All lanes : Anti-Dematin antibody (ab226357) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 46 kDa


    Exposure time: 75 seconds


    Cell lysates were prepared using NETN lysis buffer.

  • Dematin was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226357 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226357 at 1 µg/ml.

    Lane 1: ab226357 IP in HepG2 whole cell lysate.

    Lane 2: Control IgG IP in HepG2 whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab226357 has not yet been referenced specifically in any publications.

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