Overview

  • Product name
    Anti-Dengue Virus E glycoprotein antibody [DE2]
    See all Dengue Virus E glycoprotein primary antibodies
  • Description
    Mouse monoclonal [DE2] to Dengue Virus E glycoprotein
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
  • Immunogen

    Dengue Virus 2 infected supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    DE2
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20.
ELISA Use at an assay dependent dilution. Has been tested in a dengue antigen capture ELISA as well as the dengue IgM capture and IgG capture ELISA.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      Dengue virus E glycoprotein is thought to be essential for membrane fusion and is also involved in binding to cellular receptors. It is the primary antigen responsible for inducing the protective immune response.
    • Database links
      • Alternative names
        • Dengue E glycoprotein antibody
        • Dengue envelope glycoprotein antibody
        • Dengue glycoprotein E antibody
        • Dengue virus envelope glycoprotein antibody
        • Dengue virus glycoprotein E antibody
        see all

      References

      This product has been referenced in:
      • Zhang J  et al. Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production. Cell Host Microbe 23:819-831.e5 (2018). Read more (PubMed: 29902443) »
      See 1 Publication for this product

      Customer reviews and Q&As

      1-2 of 2 Abreviews or Q&A

      Answer

      Thank you for your enquiry. Basically, the epitopes for DE1 and DE2 are complex, conformational epitopes and probably non-linear - and since the immunogen is not a peptide, the amino acid sequence is not known. The information on the recombinant DIII coated plate is just additional information. Both monoclonal antibodies work with native antigens and may work with recombinant proteins which are folded properly. It may be that our recombinant DIII has not folded perfectly and therefore, both monoclonal antibodies did not work with our recombinant DIII. Unfortunately, there is no further information on this failed testing. I am sorry I can't provide more information as there is no more info on the Domain III testing results. If you have any other questions pertaining to ab41349 or ab41696, then please do not hesitate to contact me. I will do my best to locate more information for you. Have a nice day.

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      Answer

      Thank you for your enquiry. DE1 (ab41349) and DE2 (ab41696) are monoclonal antibodies from two different fusion experiments but the immunizing protocol is the same for both fusions. As mentioned in the datasheets, although they are both dengue group specific, DE1 is the monoclonal that is currently being used in the MAC-ELISA for detection of dengue-specific IgM. We believe that DE2 will function just as well in the MAC-ELISA but extensive comparisons have not been carried out. Additionally, we have tested them on recombinant DIII coated plates and they were not reactive. I hope this information will be useful. If there is anything else that I can help you with, please do not hesitate to contact me.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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