Key features and details
- Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Dengue virus 2
- Isotype: IgG1
Product nameAnti-Dengue Virus NS1 glycoprotein antibody [DN2]
See all Dengue Virus NS1 glycoprotein primary antibodies
DescriptionMouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein
SpecificityRecognises NS1 from both Dengue Virus 2 strains, 16681 and NGC.
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Species reactivityReacts with: Dengue virus 2
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab41623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/5 - 1/20.|
RelevanceNS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
- SwissProt: P29990 Dengue virus 2
- Dengue NS1 antibody
- Dengue Virus non structural protein 1 antibody
ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ab41623 staining Dengue Virus NS1 glycoprotein in HeLa cells at 62hpi with DV2 by Flow Cytometry. Cells were fixed and permeabilized with BD CytoFix/CytoPerm. The sample was incubated with the primary antibody (1/80 in CytoFix/CytoPerm) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/150) was used as the secondary antibody.
Gating Strategy: Single cells by FSC and SSC.
All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)
Predicted band size: 40 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.
ab41623 has been referenced in 6 publications.
- Yao X et al. Tatanan A from the Acorus calamus L. root inhibited dengue virus proliferation and infections. Phytomedicine 42:258-267 (2018). WB . PubMed: 29655694
- Durbin AF et al. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio 7:N/A (2016). PubMed: 27651356
- Scaturro P et al. Dengue Virus Non-structural Protein 1 Modulates Infectious Particle Production via Interaction with the Structural Proteins. PLoS Pathog 11:e1005277 (2015). PubMed: 26562291
- Immenschuh S et al. Antibodies against dengue virus nonstructural protein-1 induce heme oxygenase-1 via a redox-dependent pathway in Human endothelial cells. Free Radic Biol Med : (2012). PubMed: 23103292
- Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF . PubMed: 21658241
- Tong XK et al. WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro. Acta Pharmacol Sin 31:585-92 (2010). Flow Cyt . PubMed: 20418898