Overview

  • Product name
    Anti-Dengue Virus NS1 glycoprotein antibody [DN2]
    See all Dengue Virus NS1 glycoprotein primary antibodies
  • Description
    Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein
  • Host species
    Mouse
  • Specificity
    Recognises NS1 from both Dengue Virus 2 strains, 16681 and NGC.
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Dengue virus 2
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    DN2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/80.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.
ICC/IF 1/5 - 1/20.

Target

  • Relevance
    NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • Database links
    • Alternative names
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    Images

    • ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • ab41623 staining Dengue Virus NS1 glycoprotein in HeLa cells at 62hpi with DV2 by Flow Cytometry. Cells were fixed and permeabilized with BD CytoFix/CytoPerm. The sample was incubated with the primary antibody (1/80 in CytoFix/CytoPerm) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/150) was used as the secondary antibody.
      Gating Strategy: Single cells by FSC and SSC.

       

      See Abreview

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)

      Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
      Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
      Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
      Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)

      Predicted band size: 40 kDa
      Observed band size: 40,80 kDa
      why is the actual band size different from the predicted?



      NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.

    References

    This product has been referenced in:
    • Yao X  et al. Tatanan A from the Acorus calamus L. root inhibited dengue virus proliferation and infections. Phytomedicine 42:258-267 (2018). WB . Read more (PubMed: 29655694) »
    • Durbin AF  et al. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio 7:N/A (2016). Read more (PubMed: 27651356) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    I recommend ab138696, which has been validated in ELISA and shown to cross react with serotypes 1, 2, 3, and 4. A link to this antibody is below:



    https://www.abcam.com/dengue-virus-ns1-glycoprotein-antibody-b1424m-ab138696.html

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (BHK)
    Specification
    BHK
    Permeabilization
    Yes - BD CytoFix/CytoPerm
    Fixative
    BD CytoFix/CytoPerm

    Lee Gehrke

    Verified customer

    Submitted Oct 11 2013

    Application
    Flow Cytometry
    Fixation
    BD CytoFix/CytoPerm
    Permeabilization
    Yes - BD CytoFix/CytoPerm
    Sample
    Human Cell (HeLa at 62hpi with DV2)
    Specification
    HeLa at 62hpi with DV2
    Gating Strategy
    single cells by FSC and SSC
    Preparation
    Cell harvesting/tissue preparation method: spatula scraping
    Sample buffer: cold PBS

    Lee Gehrke

    Verified customer

    Submitted Oct 07 2013

    Answer

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in ELISA yet.

    Therefore, I can offer a discount off a future purchase if your customerbuysone of them now, tests it in ELISA and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value ofthe antibodyand can be used for the next order of any product.

    If your customer is interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and confirm which one of these antibodiesyour customer would like to testin ELISA . I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

    2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if teh antibody turns out to be unsuitable for ELISA, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glycoprotein antibody [DN3]):

    https://www.abcam.com/index.html?datasheet=41616 (or use the following: https://www.abcam.com/index.html?datasheet=41616).

    Heaton NS et al. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107:17345-50 (2010). ICC/IF. PubMed: 20855599

    Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
    https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

    ab41623 (Anti-Dengue Virus NS1 glycoprotein antibody [DN2]):

    https://www.abcam.com/index.html?datasheet=41623 (or use the following: https://www.abcam.com/index.html?datasheet=41623).

    Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF. PubMed: 21658241

    Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
    https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

    ab41490 (Anti-Dengue Virus NS1 glycoprotein antibody [DN1]):

    https://www.abcam.com/index.html?datasheet=41490 (or use the following: https://www.abcam.com/index.html?datasheet=41490).

    Currently we do not know of any references using this antibody.

    Isotype control: ab18449 (Mouse IgG2a, kappa [MOPC-173] (low endotoxin) - isotype control)
    https://www.abcam.com/index.html?datasheet=18449 (or use the following: https://www.abcam.com/index.html?datasheet=18449).

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

    For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    We do not sell directly to Pakistan. Please contact our trusted distributor for pricing information:

    Friends Scientific Corporation
    3-B Ali Continental,
    1-Mozang Road
    Lahore
    54000
    Pakistan

    Tel 4237236176 Fax 4237321500 Email mailto:friends1@wol.net.pk

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

    Answer

    Thank you for your reply. These three antibodies are provided as tissue culture supernatant. This is stated in the purity section of the datasheet. It has some sodium azide added to it, but is not provided in a storage buffer. I am sorry we do not know the concentration of these antibodies. Antibody concentration is usually determined by a protein assay. Because the tissue culture supernatant contains many other proteins, this would not be an accurate method in this case. We can estimate that tissue culture supernatantusually containsapproximately 1 - 3 mg/ml or antibody. I am sorry we are unable to provide more precise information on this occasion. However, I hope it is helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Answer

    Thank you for your enquiry. Wecan only confirm that ab41490 DN1 antibody will recognize NS1 protein from an MVA NS1 recombinant.I am sorry, we have no data from NS1 expressed in E coli or P pastoris However, if your recombinant proteins (ie NS1 from DENV2 strains 16681 or NGC) are folded properly,these antibodies would most likely work. Hence, these antibodies could be used to monitor proper folding of recombinant proteins. However, I am sorry we would not be able to guarantee this, and you would need to test this out yourself. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Answer

    Thank you for contacting Abcam. As ab41623 is a tissue culture supernatant, the concentration has not been determined. The following dilutions have been recommended, for western blot 1/50-1-100 and for ICC/IF, 1/5-1/20. The antibody has not been tested in IP and so the optimal dilution has not been determined but I would recommend starting at the higher end and using a 5ul of antibody per 100ug of tissue/cell lysate. You may find that you would have to increase this concentration but it is a good starting point. Please let me know if there is anything else I can help with.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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