Overview

  • Product name

    Anti-Dengue Virus prM glycoprotein antibody [DM1]
    See all Dengue Virus prM glycoprotein primary antibodies
  • Description

    Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
  • Host species

    Mouse
  • Specificity

    This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Dengue virus 2
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.065% Sodium azide
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    DM1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20.
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
  • Alternative names

    • pre membrane glycoprotein antibody
    • pre-membrane glycoprotein antibody

Images

  • All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

    Lanes 1-2 : Dengue Virus 1 infected C6/36 cell lysate
    Lanes 3-4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lanes 5-6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lanes 7-8 : Dengue Virus 3 infected C6/36 cell lysate
    Lanes 9-10 : Dengue Virus 4 infected C6/36 cell lysate

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

References

This product has been referenced in:

  • Saisawang C  et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13:e0193133 (2018). Read more (PubMed: 29470500) »
  • Jiang L & Sun Q The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody-dependent enhancement of infection with dengue virus serotype 3. Virol J 15:50 (2018). Read more (PubMed: 29566761) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Question
Answer

Q1. What cell type was infected with the virus in order to produce the immunogen?
A1: The mosquito cell line, C6/36, is used for growing virus.

Q2. How was the immunogen purified?
A2: Infected supernatant and lysate were used as immunogen. No purified protein was used.

Q3. Does this antibody bind to the PR peptide or to the mature protein?
A3: Sorry but we do not have any information on the specific epitope recognized by this clone. We do know that it seems the conformational epitope is mostly lost in the western blot which likely explains why DM1 reacts to all 4 dengue serotypes in a sandwich ELISA but only to dengue 2 in the western blot.


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Question
Answer



J’ai contacté le laboratoire qui m’a envoyé les résultants ELISA, voir pièce jointe.

Pour le western blot, les lysats de cellules C6/36 infectés ont été dilués dans un tampon non réducteur contenant de l’SDS-PAGE. Les échantillons n’ont pas été chauffés afin de minimiser la perte d'épitopes conformationnels.

Nous pensons que la quantité de prM dans la préparation du lysat peut être un problème ici et que la seule façon de confirmer la réactivité contre tous les 4 sérotypes de la dengue serait d'utiliser des protéines recombinantes prM correctement repliées (que nous n'avons pas).

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Answer

Thank you for your enquiry. This antibody recognizes all 4 serotypes in ELISA, however in Western blot application it detects only Dengue 2. I hope this information will be useful for you.

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