Product nameAnti-Dengue Virus prM glycoprotein antibody [DM1]
See all Dengue Virus prM glycoprotein primary antibodies
DescriptionMouse monoclonal [DM1] to Dengue Virus prM glycoprotein
SpecificityThis antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Dengue virus 2
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.065% Sodium azide
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab41473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/5 - 1/20.|
|WB||1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|ELISA||Use at an assay dependent dilution.|
RelevanceThere are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.
- pre membrane glycoprotein antibody
- pre-membrane glycoprotein antibody
All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Lanes 1-2 : Dengue Virus 1 infected C6/36 cell lysate
Lanes 3-4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lanes 5-6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lanes 7-8 : Dengue Virus 3 infected C6/36 cell lysate
Lanes 9-10 : Dengue Virus 4 infected C6/36 cell lysate
Predicted band size: 21 kDa
Observed band size: 21 kDa
Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).
This product has been referenced in:
- Saisawang C et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13:e0193133 (2018). Read more (PubMed: 29470500) »
- Jiang L & Sun Q The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody-dependent enhancement of infection with dengue virus serotype 3. Virol J 15:50 (2018). Read more (PubMed: 29566761) »