Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/250 - 1/500. Predicted molecular weight: 220 kDa.Can be blocked with DENN peptide (ab8384).|
FunctionPlays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
Tissue specificityHighly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
Sequence similaritiesBelongs to the MADD family.
Contains 1 dDENN domain.
Contains 1 death domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.
- Information by UniProt
- Differentially expressed in normal and neoplastic cells antibody
- FLJ35600 antibody
- IG20 antibody
All lanes : Anti-DENN antibody (ab2043) at 1/250 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Predicted band size: 220 kDa
ab2043 at 10µg/ml staining DENN in human spleen tissue by ICC
Immunofluorescence of MADD in Hela cells using ab2043 at 20 ug/ml.
ab2043 has not yet been referenced specifically in any publications.